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ID 109346
タイトル別表記
LED光照射による大腸癌細胞制御に関する研究
Effect of LED on Colon Cancer Cells
著者
一瀬, 規子 徳島大学大学院医科学教育部(医学専攻)
栗田, 信浩 The University of Tokushima KAKEN研究者をさがす
佐藤, 宏彦 The University of Tokushima
東島, 潤 The University of Tokushima KAKEN研究者をさがす
近清, 素也 The University of Tokushima
江藤, 祥平 The University of Tokushima
キーワード
Light-emitting diode
colon cancer
HT29
HCT116 cells
資料タイプ
学位論文
抄録
Background/Aim: Recent studies have demonstrated the efficacy of irradiation from light emitting diodes (LED) for wound healing, anti-inflammation and anticancer therapies. However, little is known about the effects of visible light in colon cancer cells. The purpose of this study was to evaluate the biological response (including gene expression changes) of human colon cancer cells to different wavelengths of LED irradiation. Materials and Methods: Human colon cancer cells (HT29 or HCT116) were seeded onto laboratory dishes that were then put on LED irradiation equipment with a 465 nm-, 525 nm-, or 635 nm-LED. Irradiation at 15 or 30 mW was performed 10 min/day, each day for 5 days. The cell counting kit8 was then used to measure cell viability. Apoptosis and expression of several mRNAs (caspase, MAPK and autophagy pathway) in HT29 cultures irradiated with 465 nm LED were evaluated via Annexin V/PI and RT-PCR, respectively. Results: Viability of HT29 and HCT116 cells was lower in 465 nm-LED irradiated cultures than in control cultures, but viability of HT29 cells did not differ between control cultures and 525 nm-LED or 635 nm-LED irradiated cultures. Moreover, the expression of FAS, caspase-3, capase-8, and JUK were significantly higher in 465 nm-LED irradiated cultures than in control cultures, and expression of ERK1/2 and LC3 was lower in blue-irradiated cells. Conclusion: LED irradiation at 465 nm inhibited the proliferation of HT29 cells and of HCT116 cells. Notably, LED irradiation at 465 nm promoted apoptosis inHT29 cultures via the extrinsic apoptosis pathway and the MAPK pathway.
掲載誌名
Anticancer Research
ISSN
02507005
17917530
cat書誌ID
AA10625860
AA12440673
出版者
The International Institute of Anticancer Research
34
9
開始ページ
4709
終了ページ
4716
発行日
2014-09-08
備考
内容要旨・審査要旨・論文本文の公開:
内容要旨・審査要旨 : LID201505141007.pdf
論文本文 : k2794_fulltext.pdf
本論文は,著者NORIKO MATSUMOTOの学位論文として提出され, 学位審査・授与の対象となっている。
著者の申請により要約(2015-05-15公開)に替えて論文全文を公開(2022-12-14)
EDB ID
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第2794号
学位記番号
甲医第1236号
学位授与年月日
2015-02-26
学位名
博士(医学)
学位授与機関
徳島大学
部局
病院
医学系
教養教育院
理工学系