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ID 110881
著者
Hemdan, Dalia Ismaeil Ibrahim Department of Nutritional Physiology, Institute of Health Biosciences, the University of Tokushima Graduate School
平坂, 勝也 Department of Nutritional Physiology, Institute of Health Biosciences, the University of Tokushima Graduate School KAKEN研究者をさがす
中尾, 玲子 Department of Nutritional Physiology, Institute of Health Biosciences, the University of Tokushima Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
コウノ, ショウヘイ Department of Nutritional Physiology, Institute of Health Biosciences, the University of Tokushima Graduate School
カガワ, サチコ Department of Nutritional Physiology, Institute of Health Biosciences, the University of Tokushima Graduate School
安倍, 知紀 Department of Nutritional Physiology, Institute of Health Biosciences, the University of Tokushima Graduate School KAKEN研究者をさがす
原田, 晃子 Department of Nutritional Physiology, Institute of Health Biosciences, the University of Tokushima Graduate School
奥村, 裕司 Department of Nutritional Physiology, Institute of Health Biosciences, the University of Tokushima Graduate School KAKEN研究者をさがす
中屋, 豊 Department of Nutritional Metabolism, Institute of Health Biosciences, the University of Tokushima Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
寺尾, 純二 Department of Food Science, Institute of Health Biosciences, the University of Tokushima Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
二川, 健 Department of Nutritional Physiology, Institute of Health Biosciences, the University of Tokushima Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
キーワード
atrogenes
dexamethasone
mouse C2C12 cells
polyphenols
3D-clinorotation
資料タイプ
学術雑誌論文
抄録
Oxidative stress is a key factor in stimulating the expression of atrogenes, which are muscle atrophy-related ubiquitin ligases, in skeletal muscle, and it induces muscle atrophy during unloading. However, the effects of antioxidative nutrients on atrogene expression have not been demonstrated. We report on the inhibitory effects of polyphenols, such as epicatechin (EC), epicatechin gallate (ECg) and epigallocatechin gallate (EGCg) and quercetin, on atrogene expression up-regulated by three dimensional (3D)-clinorotation or glucocorticoid. These treatments markedly elevated the expression of atrogenes, including atrogin-1 and MuRF-1, in mouse C2C12 myoblasts and myotubes. Interestingly, EC, ECg, EGCg and quercetin significantly decreased the expression of atrogin-1 and MuRF-1 up-regulated by 3D-clinorotation, whereas they hardly affected atrogene expression induced by dexamethasone. ERK signaling is a well known MAPK pathway to mediate oxidative stress. Therefore, we also investigated the effect of these polyphenols on phosphorylation of ERK in C2C12 myotubes. As expected, EC, ECg, EGCg, and quercetin significantly suppressed phosphorylation of ERK, corresponding to the up-regulation of atrogenes induced by 3D-clinorotation. These results suggest that antioxidative nutrients, such as catechins and quercetin, suppress atrogene expression in skeletal muscle cells, possibly through the inhibition of ERK signaling. Thus, catechins and quercetin may prevent unloading-mediated muscle atrophy.
掲載誌名
The journal of medical investigation : JMI
ISSN
13431420
cat書誌ID
AA11166929
56
1-2
開始ページ
26
終了ページ
32
並び順
26
発行日
2009-02
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
医学系