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ID 109398
タイトル別表記
C2C12筋管細胞においてフラボンはLPS誘導性atrogin-1/MAFbxの発現を抑制する
Flavones Prevent LPS-Induced Muscle Atrophy
著者
塩田, 智子 徳島大学大学院口腔科学教育部(口腔科学専攻)
Ohno, Ayako Tokushima University
キーワード
flavones
LPS
muscle atrophy
JNK
資料タイプ
学位論文
抄録
Muscle atrophy is a complex process that occurs as a consequence of various stress events. Muscle atrophy-associated genes (atrogenes) such as atrogin-1/MAFbx and MuRF-1 are induced early in the atrophy process, and the increase in their expression precedes the loss of muscle weight. Although antioxidative nutrients suppress atrogene expression in skeletal muscle cells, the inhibitory effects of flavonoids on inflammation-induced atrogin-1/MAFbx expression have not been clarified. Here, we investigated the inhibitory effects of flavonoids on lipopolysaccharide (LPS)-induced atrogin-1/MAFbx expression. We examined whether nine flavonoids belonging to six flavonoid categories inhibited atrogin-1/MAFbx expression in mouse C2C12 myotubes. Two major flavones, apigenin and luteolin, displayed potent inhibitory effects on atrogin-1/MAFbx expression. The pretreatment with apigenin and luteolin significantly prevented the decrease in C2C12 myotube diameter caused by LPS stimulation. Importantly, the pretreatment of LPS-stimulated myoblasts with these flavones significantly inhibited LPS-induced JNK phosphorylation in C2C12 myotubes, resulting in the significant suppression of atrogin-1/MAFbx promoter activity. These results suggest that apigenin and luteolin, prevent LPS-mediated atrogin-1/MAFbx expression through the inhibition of the JNK signaling pathway in C2C12 myotubes. Thus, these flavones, apigenin and luteolin, may be promising agents to prevent LPS-induced muscle atrophy.
掲載誌名
Journal of Nutritional Science and Vitaminology
ISSN
18817742
03014800
cat書誌ID
AA00703822
出版者
The Vitamin Society of Japan|Japan Society of Nutrition and Food Science|Center for Academic Publications Japan
61
2
開始ページ
188
終了ページ
194
発行日
2015-06-08
備考
内容要旨・審査要旨・論文本文の公開:
内容要旨 : LID201505281004.pdf
審査要旨 : LID201505281005.pdf
論文本文 : LID201506091001.pdf
本論文は, 著者Chieko SHIOTAの学位論文として提出され, 学位審査・授与の対象となっている。
権利情報
© The Vitamin Society of Japan, Japan Society of Nutrition and Food Science
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第2813号
学位記番号
甲口第395号
学位授与年月日
2015-03-23
学位名
博士(歯学)
学位授与機関
徳島大学
部局
医学系
歯学系
病院