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ID 116076
タイトル別表記
Chlorogenic and caffeic acid supplementation during sperm freezing
著者
Namula, Zhao Tokushima University|Guangdong Ocean University
Wittayarat, Manita Prince of Songkla University
Nguyen, Nhien Thi Tokushima University
Hirano, Takayuki Tokushima University
Nii, Masahiro Tokushima Prefectural Livestock Research Institute
キーワード
antioxidants
boar semen
caffeic acid
chlorogenic acid
sperm freezing
資料タイプ
学術雑誌論文
抄録
Chlorogenic acid (CGA) and caffeic acid (CA) are potent antioxidants that are mostly found in coffee beans. This study aimed to investigate the effects of CGA and CA supplementation during semen freezing on the quality of frozen-thawed boar spermatozoa. The antioxidants CGA and CA were added to a semen extender to achieve final concentrations of 50, 100, 200 and 400 µM. Supplementation of 100 µM CGA and CA yielded a significantly higher percentage of sperm viability (increased by 8 - 10%) and plasma membrane integrity (increased by 4 - 6%) than the control groups without the antioxidants at 0 h and 3 h after thawing (P < 0.05). At a concentration of 100 µM, CGA and CA also yielded beneficial effects on total and progressive sperm motility. Increases of CGA and CA concentrations to more than 200 µM did not enhance any sperm quality parameters. When the sperm penetrability and oocyte development by spermatozoa frozen with CGA and CA were evaluated, CGA and CA supplementations had no positive effects on the percentages of total fertilization, monospermic fertilization, cleavage and blastocyst formation. In conclusion, the supplementation of 100 µM CGA and CA during sperm freezing improved certain sperm parameters including motility, viability and plasma membrane integrity.
掲載誌名
Reproduction in Domestic Animals
ISSN
14390531
出版者
Wiley-VCH
53
6
開始ページ
1600
終了ページ
1604
発行日
2018-07-27
備考
This is the peer reviewed version of the following article: Namula, Z, Hirata, M, Wittayarat, M, et al. Effects of chlorogenic acid and caffeic acid on the quality of frozen-thawed boar sperm. Reprod Dom Anim. 2018; 53: 1600– 1604., which has been published in final form at https://doi.org/10.1111/rda.13288. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
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出版社版DOI
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フルテキストファイル
言語
eng
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著者版
部局
生物資源系