ID 110800
著者
梅野, 真由美 Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima Graduate School|Major in Laboratory Science, School of Health Sciences, Faculty of Medicine, The University of Tokushima|Core Research for Evolutional Science and Technology Corporation
新家, 利一 Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima Graduate School|Core Research for Evolutional Science and Technology Corporation
佐藤, 陽一 Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima Graduate School|Core Research for Evolutional Science and Technology Corporation 徳島大学 教育研究者総覧 KAKEN研究者をさがす
Yang, Xin-Jun Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima Graduate School|Core Research for Evolutional Science and Technology Corporation
馬場, 嘉信 Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, The University of Tokushima|Department of Applied Chemistry, Graduate School of Engineering, The University of Nagoya
イワモト, テルアキ Department of Urology, St.Marianna Medical University School of Medicine|Core Research for Evolutional Science and Technology Corporation
中堀, 豊 Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima Graduate School|Core Research for Evolutional Science and Technology Corporation
キーワード
Y chromosome
AZF
multiplex PCR
microchip electrophoresis
azoospermia
資料タイプ
学術雑誌論文
抄録
Around 10% of males with idiopathic azoospermia or oligozoospermia, which are important causes of male infertility, have partial deletions on the long arm of the Y chromosome. To develop a rapid and accurate detection system for screening major Y deletions found in infertile men, we developed a multiplex PCR system that can simultaneously amplify five loci on the Y chromosome, SRY, AMELY, DBY, RBMY, DAZ and one locus on the X chromosome, AMELX. The size of the PCR products was designed to increase gradually from the distal Yp to the distal Yq. Our system could detect deletions of three major candidate regions for the azoospermic factor, AZFa, AZFb and AZFc on the Y chromosome together with sex. The gradual increase in the size of the PCR products was convenient for imaging the location of deletions on the Y chromosome. Moreover, the multiplex PCR system was combined with microchip-based electrophoresis, and the PCR products derived from each locus were separated within 4min. Our system is useful for screening Y chromosomes bearing the structural anomalies including three major AZF deletions found among azoospermic or oligozoospermic males.
掲載誌名
The journal of medical investigation : JMI
ISSN
13431420
cat書誌ID
AA11166929
53
1-2
開始ページ
147
終了ページ
152
並び順
147
発行日
2006-02
EDB ID
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
薬学系