Archaea ATP-Dependent RNA Ligase
Liu, Yancheng University of Tsukuba
Takagi, Yuko National Institute of Advanced Industrial Science and Technology
Sugijanto, Milyadi University of Tsukuba
Duong My Nguyen, Kieu University of Tsukuba
Hori, Hiroyuki Ehime University
Ho, C. Kiong University of Tsukuba
thermococcus kodakarensis KOD1
C/D box sRNAs
RNA ligases play important roles in repairing and circularizing RNAs post-transcriptionally. In this study, we generated an allelic knockout of ATP-dependent RNA ligase (Rnl) in the hyperthermophilic archaeon Thermococcus kodakarensis to identify its biological targets. A comparative analysis of circular RNA reveals that the Rnl-knockout strain represses circularization of C/D box sRNAs without affecting the circularization of tRNA and rRNA processing intermediates. Recombinant archaeal Rnl could circularize C/D box sRNAs with a mutation in the conserved C/D box sequence element but not when the terminal stem structures were disrupted, suggesting that proximity of the two ends could be critical for intramolecular ligation. Furthermore, T. kodakarensis accumulates aberrant RNA fragments derived from ribosomal RNA in the absence of Rnl. These results suggest that Rnl is responsible for C/D box sRNA circularization and may also play a role in ribosomal RNA processing.
Frontiers in Molecular Biosciences
Frontiers Media S.A.
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