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ID 114526
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乳癌捺印細胞診検体のHER2およびKi67免疫染色は組織診断に代替可能である
著者
森本, 雅美 The University of Tokushima
中川, 美砂子 The University of Tokushima KAKEN研究者をさがす
武知, 浩和 The University of Tokushima KAKEN研究者をさがす
吉田, 卓弘 The University of Tokushima KAKEN研究者をさがす
本田, 純子 Higashi Tokushima Medical Center
田所, 由紀子 The University of Tokushima KAKEN研究者をさがす
Moriya, Takuya Kawasaki Medical School
Sasa, Mitsunori Tokushima Breast Care Clinic
キーワード
Breast cancer
HER2
Ki67
Immunocytochemical analysis
Touch-smear cells
資料タイプ
学位論文
抄録
Background: Re-evaluation of the subtype of recurrent breast cancer is necessary for deciding the treatment approach, but it is often not performed due to the difficulty of obtaining tissue specimens from a recurrent lesion, etc. However, when a recurrent lesion is close to the body surface, fine-needle aspiration cells (FNA cells) can be easily obtained, and immunocytochemical (ICC) analysis of hormone receptors expression in FNA cells is said to be highly reliable. However, there is no consensus regarding ICC analysis of human epidermal growth factor receptor type2 (HER2) expression and the Ki67 index using FNA cells.
Methods: Touch-smear cells (TSC) were prepared from resected specimens from 36 patients with primary invasive ductal carcinoma of the breast. The TSC were fixed in 95% ethanol and subjected to ICC analysis for HER2 using HercepTestTM (Dako) and Ki67 using MIB-1TM (Dako). HER2 expression and the Ki67 index for the TSC were compared with the results of immunohistochemical analysis of histological section (HS). Statistical analyses used the kappa test and Pearson’s correlation coefficients.
Results: HER2 and Ki67 were analyzed in TSC from 36 and 28 patients, respectively. The HER2 expression scores in the TSC and HS groups showed good agreement (kappa value=0.640), and significant correlation (correlation coefficient=0.860, p<0.001). The Ki67 indexes in the TSC and HS groups also showed significant correlation (correlation coefficient=0.861, p<0.001).
Conclusions: The reliability of ICC analysis of HER2 expression and the Ki67 index using TSC were recognized.
掲載誌名
Breast Cancer
ISSN
13406868
18804233
cat書誌ID
AA1103354X
出版者
Springer Nature|The Japanese Breast Cancer Society
23
4
開始ページ
577
終了ページ
582
発行日
2015-03-26
備考
内容要旨・審査要旨・論文本文の公開
本論文は,著者Masami Morimotoの学位論文として提出され,学位審査・授与の対象となっている。
This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: https://doi.org/10.1007/s12282-015-0605-z
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
乙第2112号
学位記番号
乙医第1760号
学位授与年月日
2020-03-18
学位名
博士(医学)
学位授与機関
徳島大学
部局
医学系
病院