Ge, Ning Third Department of Internal Medicine, The University of Tokushima School of Medicine
ナカムラ, ヨウイチ Third Department of Internal Medicine, The University of Tokushima School of Medicine
中屋, 豊 Department of Nutrition, The University of Tokushima School of Medicine 徳島大学 教育研究者総覧 KAKEN研究者をさがす
outwardly rectifying chloride channel
cystic fibrosis transmembrane conductance regulator
The mechanism of increased chloride currents by inflammatory cytokine, interferon-gamma (IFN-γ), was investigated in cultured a human bronchial epithelial cell line (BEAS-2B) using cell-attached and inside-out patch configurations. The channel sensitive to chloride ion was activated by forskolin, an activator of adenylate cyclase, or 100μM dibutyryl 5'-cyclic monophosphate in cell-attached configurations. The conductance of this channel was 40±4 pS in symmetrical 150 mM chloride solution between membrane potentials of 0 to +50 mV, and this channel was blocked by 500μM 4,4'-diisothiocyanatostilbene-2, 2'-disulfonic acid (DIDS), suggesting that this channel was an outwardly rectifying chloride channel (ORCC). Treatment of 10-1000 U/ml IFN-γ for 3 hours, but not IFN-α, significantly increased channel activities of ORCC, and this activation was observed at least 24 hours after treatment. Erythromycin, a macrolide antibiotic, at a concentration of100μM inhibited the activation of ORCC induced by IFN-γ. The findings of the present study indicate that increased mucus secretion during inflammation might be partly due to activation of chloride permeability by cytokine and erythromycin might improve oversecretion of mucus from bronchial epithelium by blocking ORCC.
The journal of medical investigation : JMI
LID201112021006.pdf 102 KB