ID | 117633 |
タイトル別表記 | Proteome of the aging mice heart
|
著者 |
Maekura, Koji
Kobe Gakuin University
Yamano, Ryo
Kobe Gakuin University
Matsuura, Kenji
Osaka Ohtani University
Kadoyama, Keiichi
Dokkyo University
Matsuyama, Shogo
Kobe University
Hamada, Michiko
Kobe Gakuin University
Takano, Masaoki
Kobe Gakuin University
|
キーワード | Proteome
phosphoprotein
heart
aging
mitochondrial protein
|
資料タイプ |
学術雑誌論文
|
抄録 | Aging induces pathological cardiovascular changes such as cardiac dysfunction and arteriosclerosis. With aging, heart cells, especially, become more susceptible to lethal damage. In this report, we tried to understand the precise mechanism of myocardial change resulting from aging by examining the heart proteome in aging mice using two-dimensional gel electrophoresis (2DE). The proteins were stained with fluorescence dyes (SYPRO Ruby and Pro-Q Diamond) and identified by subsequent MALDI-TOF-MS / MS. As a result, markedly altered levels of 14 proteins and 7 phosphoproteins were detected in the hearts of 3-, 7-, 11-, and 20-month-old mice. The functions of these identified proteins and phosphoproteins were energy metabolism, muscle contraction, glycolysis, and cytoskeletal support. Additionally, the results of Western blotting confirmed changes in the expression of FTH, CPNE5, and SUCLA2. These findings showed that aging modified the expression of proteins and phosphoproteins in the heart. We suggest that changes in the expression of these proteins are critical to the development of cardiac dysfunction resulting from aging.
|
掲載誌名 |
The Journal of Medical Investigation
|
ISSN | 13496867
13431420
|
cat書誌ID | AA11166929
|
出版者 | Tokushima University Faculty of Medicine
|
巻 | 69
|
号 | 3-4
|
開始ページ | 217
|
終了ページ | 223
|
並び順 | 217
|
発行日 | 2022-08
|
出版社版DOI | |
出版社版URL | |
フルテキストファイル | |
言語 |
eng
|
著者版フラグ |
出版社版
|