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ID 114734
タイトル別表記
単球由来線維細胞のパラクライン効果を介した線維化促進作用の機能解析
Fibrocytes activate fibroblasts by PDGF.
著者
阿部, 秀一 徳島大学大学院医科学教育部(医学専攻)
Kishi, Masami Tokushima University
Koyama, Kazuya Tokushima University
Takahashi, Naoki Tokushima University
キーワード
fibrocyte
fibroblast
idiopathic pulmonary fibrosis
PDGF
資料タイプ
学位論文
抄録
Fibrocytes, which are bone marrow-derived collagen-producing cells, were reported to play a role in the pathogenesis of pulmonary fibrosis. However, their function in pulmonary fibrosis is unclear. We analyzed their function compared with that of monocytes and localization in fibrotic tissues in patients with idiopathic pulmonary fibrosis (IPF). We compared the gene expression profile of monocyte-derived fibrocytes with that of monocytes by microarray analysis. Proliferation and differentiation into myofibroblasts were examined by 3H-thymidine incorporation assay and Western blotting. We measured the level of growth factors in the culture supernatant of fibrocytes by ELISA. The localization of fibrocytes in lung tissues of patients with IPF was determined by immunofluorescence staining. Compared with monocytes, fibrocytes had higher expression of extracellular matrix- and growth factor-encoding genes, including PDGF-B, FGF-2 and VEGF-B. Although fibrocytes did not proliferate in response to PDGF, co-culture of fibrocytes stimulated the growth of lung fibroblasts through the production of PDGF-BB. In the lung of IPF patients, CD45+Collagen-I+FSP-1+ cells, which have a similar phenotype to fibrocytes, were detected and co-stained with anti-PDGF antibody. This study suggested that fibrocytes function in pulmonary fibrosis partly by producing PDGF in the lungs of IPF patients.
掲載誌名
The Journal of Medical Investigation
ISSN
13496867
13431420
cat書誌ID
AA12022913
AA11166929
出版者
Tokushima University Faculty of Medicine
67
1-2
開始ページ
102
終了ページ
112
並び順
102
発行日
2020-02
備考
内容要旨・審査要旨・論文本文の公開
本論文は, 著者Shuichi Abeの学位論文として提出され, 学位審査・授与の対象となっている。
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第3363号
学位記番号
甲医第1446号
学位授与年月日
2020-03-05
学位名
博士(医学)
学位授与機関
徳島大学
部局
病院
医学系