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ID 115720
著者
Hasan, Mahadi Kyoto Pharmaceutical University
Fujikawa, Koki Tokushima University
Ohgita, Takashi Kyoto Pharmaceutical University
Hama, Susumu Kyoto Pharmaceutical University
斎藤, 博幸 Kyoto Pharmaceutical University KAKEN研究者をさがす
キーワード
iRed
4′-thioDNA
RNAi effect
faint electric treatment
cytoplasmic delivery
資料タイプ
学術雑誌論文
抄録
An intelligent shRNA expression device (iRed) contains the minimum essential components needed for shRNA production in cells, and could be a novel tool to regulate target genes. However, general delivery carriers consisting of cationic polymers/lipids could impede function of a newly generated shRNA via electrostatic interaction in the cytoplasm. Recently, we found that faint electric treatment (fET) of cells enhanced delivery of siRNA and functional nucleic acids into the cytoplasm in the absence of delivery carriers. Here, we examined fET of cells stably expressing luciferase in the presence of iRed encoding anti-luciferase shRNA. Transfection of lipofectamine 2000 (LFN)/iRed lipoplexes showed an RNAi effect, but fET-mediated iRed transfection did not, likely because of the endosomal localization of iRed after delivery. However, fET in the presence of lysosomotropic agent chloroquine significantly improved the RNAi effect of iRed/fET to levels that were higher than those for the LFN/iRed lipoplexes. Furthermore, the amount of lipid droplets in adipocytes significantly decreased following fET with iRed against resistin in the presence of chloroquine. Thus, iRed could be a useful tool to regulate target genes following fET-mediated cytoplasmic delivery with endosomal escape devices.
掲載誌名
Science and Technology of Advanced Materials
ISSN
14686996
18785514
cat書誌ID
AA11561821
AA11539797
出版者
Taylor & Francis|National Institute for Materials Science
17
1
開始ページ
554
終了ページ
562
発行日
2016-09-16
権利情報
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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言語
eng
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出版社版
部局
薬学系