ID 25703
著者
イケダ, ユウイチ Department of Orthopedic Surgery, The University of Tokushima School of Medicine
イカタ, タカアキ Department of Orthopedic Surgery, The University of Tokushima School of Medicine
ミシロ, タクヤ Department of Orthopedic Surgery, The University of Tokushima School of Medicine
中野, 俊次 Department of Orthopedic Surgery, The University of Tokushima School of Medicine
イケベ, ミツグ Department of Orthopedic Surgery, The University of Tokushima School of Medicine
ヤスオカ, ススム Department of Nursing, The University of Tokushima School of Medical Sciences
キーワード
synovial fluid
cysteine protease
cathepsin B
rheumatoid arthritis
pro-urokinase
資料タイプ
学術雑誌論文
抄録
To clarify the pathophysiological role of cathepsins in rheumatoid arthritis (RA), we investigated whether cathepsin B or cathepsin L was increased in synovial fluid (SF) of RA joints, and whether the cathepsin isolated from SF of RA patients activated pro-urokinase or not. Thus, we estimated the content of cathepsins in SF of RA patients by measuring their activities by fluorospectrometry, using Z-Phe-Arg-MCA as the substrate. Cathepsin activity was approxymately 4-fold higher in the SF of RA patients than in those of patients with osteoarthritis. Cathepsin B and cathepsin L were separated by cation-exchange column chromatography. As a result, a large peak corresponding to cathepsin B and a very small peak correponding to cathepsin L were detected.
Biochemical sequential fractionation of the cathepsin purified from the SF showed that the large peak was mainly composed of cathepsin B. This purified enzyme induced conversion of pro-urokinase to urokinase, and the Km for pro-urokinase was approximately 8.27μM.
These findings indicated that an imbalance between cathepsin B and its inhibitors occurred due to increased concentrations of active cathepsin B in RA articular lesions, and that cathepsin B might be related to the degradation of cartilage in RA by activating the fibrinolytic cascade.
掲載誌名
The journal of medical investigation : JMI
ISSN
13431420
cat書誌ID
AA11166929
47
1-2
開始ページ
61
終了ページ
75
並び順
61
発行日
2000
備考
EDB ID
フルテキストファイル
言語
eng