Establishment of Immunodeficient Retinal Degeneration Model Mice and Functional Maturation of Human ESC-Derived Retinal Sheets after Transplantation

Iraha, Satoshi; Tu, Hung-Ya; Yamasaki, Suguru; Kagawa, Takahiro; Goto, Motohito; Takahashi, Riichi; Watanabe, Takehito; Sugita, Sunao; Yonemura, Shigenobu; Sunagawa, Genshiro A.; Matsuyama, Take; Fujii, Momo; Kuwahara, Atsushi; Kishino, Akiyoshi; Koide, Naoshi; Eiraku, Mototsugu; Tanihara, Hidenobu; Takahashi, Masayo; Mandai, Michiko

doi:10.1016/j.stemcr.2018.01.032

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ID	115734
著者	Iraha, Satoshi RIKEN\|Kumamoto University\|Kyoto University Tu, Hung-Ya RIKEN Yamasaki, Suguru RIKEN\|Sumitomo Dainippon Pharma Kagawa, Takahiro Animal Resources and Technical Research Center Goto, Motohito Animal Resources and Technical Research Center Takahashi, Riichi Animal Resources and Technical Research Center Watanabe, Takehito RIKEN Sugita, Sunao RIKEN 米村, 重信 RIKEN\|Tokushima University 徳島大学教育研究者総覧 KAKEN研究者をさがす Sunagawa, Genshiro A. RIKEN Matsuyama, Take RIKEN Fujii, Momo RIKEN Kuwahara, Atsushi Sumitomo Dainippon Pharma Kishino, Akiyoshi Sumitomo Dainippon Pharma Koide, Naoshi RIKEN Eiraku, Mototsugu RIKEN Tanihara, Hidenobu Kumamoto University Takahashi, Masayo RIKEN\|Kyoto University Mandai, Michiko RIKEN
資料タイプ	学術雑誌論文
抄録	Increasing demand for clinical retinal degeneration therapies featuring human ESC/iPSC-derived retinal tissue and cells warrants proof-of-concept studies. Here, we established two mouse models of end-stage retinal degeneration with immunodeficiency, NOG-rd1-2J and NOG-rd10, and characterized disease progress and immunodeficient status. We also transplanted human ESC-derived retinal sheets into NOG-rd1-2J and confirmed their long-term survival and maturation of the structured graft photoreceptor layer, without rejection or tumorigenesis. We recorded light responses from the host ganglion cells using a multi-electrode array system; this result was consistent with whole-mount immunostaining suggestive of host-graft synapse formation at the responding sites. This study demonstrates an application of our mouse models and provides a proof of concept for the clinical use of human ESC-derived retinal sheets.
掲載誌名	Stem Cell Reports
ISSN	22136711
出版者	International Society for Stem Cell Research\|Cell Press
巻	10
号	3
開始ページ	1059
終了ページ	1074
発行日	2018-03-01
権利情報	This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
EDB ID	355254
出版社版DOI	10.1016/j.stemcr.2018.01.032
出版社版URL	https://doi.org/10.1016/j.stemcr.2018.01.032
フルテキストファイル	stemcr_10_3_1059.pdf 7.16 MB
言語	eng
著者版フラグ	出版社版
部局	医学系