佐埜, 弘樹 徳島大学大学院医科学教育部（医学専攻）
Namekata, Kazuhiko Tokyo Metropolitan Institute of Medical Science
Kimura, Atsuko Tokyo Metropolitan Institute of Medical Science
Shitara, Hiroshi Tokyo Metropolitan Institute of Medical Science
Guo, Xiaoli Tokyo Metropolitan Institute of Medical Science
Harada, Chikako Tokyo Metropolitan Institute of Medical Science
Harada, Takayuki Tokyo Metropolitan Institute of Medical Science|Tokushima University
N-acetylcysteine (NAC) is widely used as a mucolytic agent and as an antidote to paracetamol overdose. NAC serves as a precursor of cysteine and stimulates the synthesis of glutathione in neural cells. Suppressing oxidative stress in the retina may be an effective therapeutic strategy for glaucoma, a chronic neurodegenerative disease of the retinal ganglion cells (RGCs) and optic nerves. Here we examined the therapeutic potential of NAC in two mouse models of normal tension glaucoma, in which excitatory amino-acid carrier 1 (EAAC1) or glutamate/aspartate transporter (GLAST) gene was deleted. EAAC1 is expressed in retinal neurons including RGCs, whereas GLAST is mainly expressed in Müller glial cells. Intraperitoneal administration of NAC prevented RGC degeneration and visual impairment in EAAC1-deficient (knockout; KO) mice, but not in GLAST KO mice. In EAAC1 KO mice, oxidative stress and autophagy were suppressed with increased glutathione levels by NAC treatment. Our findings suggest a possibility that systemic administration of NAC may be available for some types of glaucoma patients.
Cell Death & Disease
本論文は, 著者Hiroki Sanoの学位論文として提出され, 学位審査・授与の対象となっている。
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