直近一年間の累計
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ID 63727
著者
Matsui, Hiroko 九州大学
Sakanashi, Yoko 九州大学
Nishimura, Yumiko 岡山大学
Kawanai, Takuya 徳島大学
Ishida, Shiro 徳島文理大学
Okano, Yoshiro 徳島文理大学
キーワード
TPEN
DTPA
zinc
hydrogen peroxide
cytotoxicity
資料タイプ
紀要論文
抄録
Recently, we have revealed that trace Zn2+ partly attenuates Ca2+-dependent cell death induced by A23187, a calcium ionophore, in rat thymocytes. In this study, to see if Zn2+ attenuates the H2O2-induced cell death that is also Ca2+-dependent, the effects of ZnCl2 and chelators for Zn2+ have been examined by using a flowcytometer with propidium iodide. The incubation with H2O2 increased the cell lethality. Simultaneous
application of ZnCl2 greatly augmented the H2O2-induced increase in lethality. DTPA, a chelator for extracellular Zn2+, did not affect the increase in cell lethality by H2O2. However, the H2O2-induced increase in cell lethality was greatly attenuated by TPEN, a chelator for extracellular and intracellular Zn2+. Taken together, it may be likely that intracellular Zn2+ modifies the H2O2-induced cytotoxicity. However, it cannot be ruled out the possibility that TPEN chelates intracellular Fe2+, resulting in inhibiting the formation of hydroxyl radical
from H2O2 that leads to an attenuation of H2O2 cytotoxicity.
掲載誌名
徳島大学総合科学部自然科学研究 = Natural Science Research, The University of Tokushima
ISSN
09146385
cat書誌ID
AN10065859
出版者
徳島大学総合科学部
23
2
開始ページ
13
終了ページ
19
並び順
13
発行日
2009-07-01
EDB ID
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
生物資源系