ID 110862
著者
三好, 圭子 Department of Molecular Biology, Institute of Health Biosciences, the University of Tokushima Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
ナガタ, ヒデヤ Department of Applied Chemistry, Graduate School of Engineering, Nagoya University
堀口, 大吾 Department of Molecular Biology, Institute of Health Biosciences, the University of Tokushima Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
阿部, 佳織 Department of Molecular Biology, Institute of Health Biosciences, the University of Tokushima Graduate School KAKEN研究者をさがす
Wahyudi, Ivan Arie Department of Molecular Biology, Institute of Health Biosciences, the University of Tokushima Graduate School
馬場, 嘉信 Department of Applied Chemistry, Graduate School of Engineering, Nagoya University
ハラダ, ヒデミツ Department of Oral Anatomy II, Iwate Medical University School of Dentistry
野間, 隆文 Department of Molecular Biology, Institute of Health Biosciences, the University of Tokushima Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
キーワード
dental epithelium
BMP2
microarray
gene profiling
資料タイプ
学術雑誌論文
抄録
Tooth development is regulated by epithelial-mesenchymal interactions and their reciprocal molecular signaling. Bone morphogenetic protein 2 (BMP2) is known as one of the inducers for tooth development. To analyze the molecular mechanisms of BMP2 on ameloblast differentiation (amelogenesis), we performed microarray analyses using rat dental epithelial cell line, HAT-7. After confirming that BMP2 could activate the canonical BMP-Smads signaling in HAT-7 cells, we analyzed the effects of BMP2 on 14,815 gene expressions and profiled them. Seventy-three genes were up-regulated and 28 genes were down-regulated by BMP2 treatment for 24 hours in HAT-7 cells. Functional classification revealed that 18% of up-regulated genes were ECM/adhesion molecules present in the enamel organ. Furthermore, we examined the expression of several differentiation markers in dental epithelial four cell-lineages including inner enamel epithelium (ameloblasts), stratum intermedium, stratum reticulum, and outer enamel epithelium. The results indicated that BMP2 might induce at least two different cell-lineage markers including a BMP antagonist expressed in HAT-7 cells, suggesting that BMP2 could accelerate amelogenesis via BMP signaling.
掲載誌名
The journal of medical investigation : JMI
ISSN
13431420
cat書誌ID
AA11166929
55
3-4
開始ページ
216
終了ページ
226
並び順
216
発行日
2008-08
EDB ID
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
歯学系
病院