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ID 115722
著者
Jabasini, Mohammad The University of Tokushima|Nagoya University
エヴィス, アシュラフ The University of Tokushima
中堀, 豊 The University of Tokushima
馬場, 嘉信 Nagoya University|National Institute of Advanced Industrial Science and Technology
キーワード
anomalous separation
DNA
microchip electrophoresis
資料タイプ
学術雑誌論文
抄録
We investigated an anomalous DNA separation where two DNA fragments from the human Y-chromosome sY638 (64 bp) and sY592 (65 bp), with only one base pair difference, were separated. This result is abnormal since in a previous study, we found that 5 bp was the minimum difference between two DNA fragments that the microchip electrophoresis system can separate. The formation of a mini-loop in the structure of the DNA fragment of sY638 (64 bp) was strongly expected to be the reason. To investigate this, we synthesized three modified DNA fragments for sY638 (64 bp), and the modifications were in two expected locations for possible mini-loop formation. Later, the separation between sY592 (65 bp) and the three modified fragments of sY638 (64 bp) was not possible. Thus, we conclude that the formation of a mini-loop in the structure of the DNA is the reason behind this anomalous separation.
掲載誌名
Scientia Pharmaceutica
ISSN
22180532
出版者
MDPI
84
3
開始ページ
507
終了ページ
513
発行日
2016-05-26
権利情報
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
薬学系