ID | 116985 |
著者 |
Namula, Zhao
Guangdong Ocean University|Tokushima University
Le, Quynh Anh
Tokushima University
Lin, Qingyi
Tokushima University
Takebayashi, Koki
Tokushima University
Yoshimura, Naoaki
Tokushima University
ソンキテイデイロ, チヨンマナート
Walailak University
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キーワード | CRISPR
electroporation
genome editing
porcine zygote
zona pellucida
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資料タイプ |
学術雑誌論文
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抄録 | Background: Increasing the permeability of the zona pellucida (ZP) of oocytes before CRISPR/Cas9 electroporation may improve the efficiency of gene editing; however, the effects of this approach on subsequent developmental processes are unclear. In this study, the effects of ZP treatment before electroporation on embryonic development and gene editing in porcine embryos were evaluated.
Methods: The ZP of zygotes was weakened or removed by exposure to 0.5% actinase E, followed by electroporation of the Cas9 protein with guide RNA targeting GGTA1. Results: The blastocyst formation rate of ZP-free zygotes after electroporation was significantly lower (p < 0.05) than that of ZP-intact zygotes. The mutation rate in blastocysts from ZP-weakened zygotes was similar to that in ZP-intact zygotes, whereas ZP removal increased the mutation rate. The mutation efficiency in blastocysts from electroporated zygotes did not differ among ZP treatment groups. Conclusions: Our results indicate that weakening the ZP does not affect the developmental competence, mutation rate, or mutation efficiency of electroporated zygotes, whereas ZP removal has a detrimental effect on embryonic development but may increase the mutation rate. |
掲載誌名 |
Veterinary Medicine and Science
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ISSN | 20531095
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出版者 | John Wiley & Sons
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巻 | 8
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号 | 1
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開始ページ | 164
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終了ページ | 169
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発行日 | 2021-10-21
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権利情報 | This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
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言語 |
eng
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出版社版
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部局 |
生物資源系
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