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ID 115749
著者
Matoba, Masaki Tokushima University
Takao, Ayuko Tsurumi University
Whiley, Robert A. Queen Mary University of London
Maeda, Nobuko Tsurumi University
キーワード
Streptococcus intermedius
intermedilysin
screening
MsgA
NanA
LacR
資料タイプ
学術雑誌論文
抄録
Streptococcus intermedius is a member of the normal human commensal flora and secretes a human-specific cytolysin intermedilysin (ILY) as a major virulence factor. Expression of ily is repressed by LacR and loss-of-function mutations of LacR are observed in many ILY high-producing strains isolated from deep-seated abscesses, suggesting that high ILY production is necessary for increased virulence. However, because ILY exhibits no β-hemolysis on animal blood agar plates, differentiating ILY high- and low-producing strains using conventional laboratory methods is not possible. Interestingly, S. intermedius also produces glycosidases, including MsgA and NanA, which exhibit N-acetyl-β-D-glucosaminidase and neuraminidase activities, respectively. Moreover, MsgA expression, but not NanA, is negatively regulated by LacR. Here we measured the activities of MsgA, NanA and ILY in strains isolated from clinical specimens and dental plaque to determine the correlation between these glycosidase activities and ILY hemolytic activity. Hemolytic activity showed a strong positive correlation with MsgA and a weak negative correlation with NanA activities. Therefore, we calculated the ratio of MsgA and NanA activity (M/N ratio). This value showed a stronger positive correlation (r = 0.81) with ILY hemolytic activity and many strains with high M/N ratios (> 2) were ILY high-producers with loss-of-function mutations in LacR.
抄録別表記
Rapid screening method for highly pathogenic S. intermedius strains based on the ratio of activities of MsgA and NanA resulting from lacR mutations.
掲載誌名
FEMS Microbiology Letters
ISSN
15746968
03781097
cat書誌ID
AA00193861
出版者
Federation of European Microbiological Societies|Oxford University Press
365
3
開始ページ
fnx258
発行日
2017-12-06
備考
This is a pre-copyedited, author-produced version of an article accepted for publication in FEMS Microbiology Letters following peer review. The version of record Toshifumi Tomoyasu, Masaki Matoba, Ayuko Takao, Atsushi Tabata, Robert A Whiley, Nobuko Maeda, Hideaki Nagamune, Rapid screening method for detecting highly pathogenic Streptococcus intermedius strains carrying a mutation in the lacR gene, FEMS Microbiology Letters, Volume 365, Issue 3, February 2018, fnx258 is available online at: https://doi.org/10.1093/femsle/fnx258
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
著者版
部局
生物資源系