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ID 117781
タイトル別表記
Development of SrtA-mediated Peptide-labeled Liposome
著者
Ohkubo, Yukimasa Tokushima University
Anyoji, Natsuki Tokushima University
Hojo, Keiko Kobe Gakuin University
Tatematsu, Youhei Suzuka University of Medical Science
大倉, 一人 Suzuka University of Medical Science
キーワード
Drug-delivery system
liposome
sortase A
資料タイプ
学術雑誌論文
抄録
Background/Aim: In order to develop an efficient drug-delivery system (DDS), a lipopeptide-loaded liposome that functions as a platform for the transpeptidase reaction mediated by sortase A (SrtA) was constructed and its stability, as well as cell-specific targeting were evaluated in the present study. Materials and Methods: Several lipopeptides possessing an acceptor peptide sequence (oligoglycine ≥ three residues) or donor peptide sequence (LPETG) for the SrtA-mediated reaction were chemically synthesized and then inserted into the liposome membrane composed of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and cholesterol (DPPC-Chol-lipo) to obtain the lipopeptide-loaded liposomes. The transpeptidase reaction mediated by recombinant SrtA (His-ΔN59SrtA) was employed to modify the peptide moiety on the liposomal surface using a fluorescently-labeled substrate peptide corresponding to the species of each loaded lipopeptide. Furthermore, lung tumor-binding peptide (LTBP)-labeled liposomes, prepared by this transpeptidase reaction, were investigated for selective targeting to lung cancer cells in vitro. Results and Discussion: The His-ΔN59SrtA-mediated transpeptidation of fluorescently-labeled peptide on the lipopeptide-loaded DPPC-Chol-lipo was confirmed. The selective targeting of LTBP-labeled liposomes to the lung cancer cell line A549 was also observed in vitro. These results suggest that the labeling of acceptor or donor lipopeptide-loaded liposomes with the transpeptidase SrtA could be a useful method for developing a platform applicable to a cancer-targeting DDS.
掲載誌名
Anticancer Research
ISSN
02507005
17917530
cat書誌ID
AA10625860
AA12440673
出版者
The International Institute of Anticancer Research
35
8
開始ページ
4411
終了ページ
4417
発行日
2015-07-10
EDB ID
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
生物資源系