直近一年間の累計
アクセス数 : ?
ダウンロード数 : ?
ID 117782
タイトル別表記
Preferred Conditions for SrtA Transpeptidation for Creating a DDS Tool
著者
Anyoji, Natsuki The University of Tokushima
Ohkubo, Yukimasa The University of Tokushima
キーワード
Sortase A
Staphylococcus aureus
drug-delivery system
liposome
資料タイプ
学術雑誌論文
抄録
Background/Aim: This study aimed to determine the preferred conditions for the transpeptidase reaction of sortase A from Staphylococcus aureus, for the purpose of creating functional liposomes useful for a drug-delivery system (DDS). Materials and Methods: His-tagged recombinant sortase A with 59 amino acids deleted from the N-terminus (His-ΔN59SrtA) was prepared using an Escherichia coli expression system. The pH dependency and sorting signal sequence dependency of the transpeptidase reaction of His-ΔN59SrtA were analyzed by monitoring the transfer of model donor-substrates (i.e. His-tagged mutant green fluorescent proteins with a C-terminal LPxTG sorting signal) to model acceptor-beads with a GGGGGC peptide. In addition, using preferred conditions, the sortase A reaction was used to modify liposome surface. Results and Discussion: The transpeptidase reaction of His-ΔN59SrtA was enhanced under weakly acidic conditions. Transfer efficiency, based on sorting signal recognition by His-ΔN59SrtA, was similar to or higher than that obtained using several substrates with amino acids other than Glu in the sorting signal position “x”. Furthermore, liposomes containing GGGGGC peptide-linked dipalmitoylphosphatidylethanolamine were successfully modified using the preferred conditions for His-ΔN59SrtA determined in this study. Conclusion: Preferred conditions for the transpeptidase reaction of His-ΔN59SrtA, especially in a weakly acidic environment to enhance reaction, was established and successfully used to create functional liposomes applicable to DDS.
掲載誌名
Anticancer Research
ISSN
02507005
17917530
cat書誌ID
AA10625860
AA12440673
出版者
The International Institute of Anticancer Research
34
8
開始ページ
4521
終了ページ
4527
発行日
2014-07-29
EDB ID
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
生物資源系