ID | 115291 |
タイトル別表記 | 歯髄幹細胞からの分泌因子は、顎関節変形性関節症の治療に多面的な効果を示す
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著者 |
小笠原, 直子
徳島大学大学院口腔科学教育部(口腔科学専攻)
Liu, Yao
Tokushima University
Xia, L.
Tokushima University
Sakamaki, T.
Tokushima University
Hibi, H.
Nagoya University
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キーワード | Dental pulp stem cell
Conditioned medium
Chondrocyte
Cartilage regeneration
Temporomandibular joint
Osteoarthritis
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資料タイプ |
学位論文
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抄録 | Objective: Temporomandibular joint osteoarthritis (TMJOA) is a degenerative disease characterized by progressive cartilage degeneration, abnormal bone remodeling, and chronic pain. In this study, we aimed to investigate effective therapies to reverse or suppress TMJOA progression.
Design: To this end, we performed intravenous administration of serum free conditioned media from human exfoliated deciduous teeth stem cells (SHED-CM) into a mechanical-stress induced murine TMJOA model. Results: SHED-CM administration markedly suppressed temporal muscle inflammation, and improved bone integrity and surface smoothness of the destroyed condylar cartilage. Moreover, SHED-CM treatment decreased the number of IL-1β, iNOS, and MMP-13 expressing chondrocytes, whereas it specifically increased PCNA-positive cells in the multipotent polymorphic cell layer. Notably, the numbers of TdT-mediated dUTP nick end labeling (TUNEL)-positive apoptotic chondrocytes in the SHED-CM treated condyles were significantly lower than in those treated with DMEM, whereas the proteoglycan positive area was restored to a level similar to that of the sham treated group, demonstrating that SHED-CM treatment regenerated the mechanical-stress injured condylar cartilage and subchondral bone. Secretome analysis revealed that SHED-CM contained multiple therapeutic factors that act in osteochondral regeneration. Conclusions: Our data demonstrated that SHED-CM treatment promoted the regeneration and repair of mechanical-stress induced mouse TMJOA. Our observations suggest that SHED-CM has potential to be a potent tissue-regenerating therapeutic agent for patients with severe TMJOA. |
掲載誌名 |
Osteoarthritis and Cartilage
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ISSN | 10634584
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cat書誌ID | AA10998176
AA11537860
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出版者 | Osteoarthritis Research Society International|Elsevier Ltd.
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巻 | 28
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号 | 6
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開始ページ | 831
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終了ページ | 841
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発行日 | 2020-04-06
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備考 | 内容要旨・審査要旨・論文本文の公開
本論文は,著者N. Ogasawaraの学位論文として提出され,学位審査・授与の対象となっている。 |
EDB ID | |
出版社版DOI | |
出版社版URL | |
フルテキストファイル | |
言語 |
eng
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著者版フラグ |
博士論文全文を含む
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文科省報告番号 | 甲第3444号
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学位記番号 | 甲口第465号
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学位授与年月日 | 2020-09-10
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学位名 |
博士(歯学)
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学位授与機関 |
徳島大学
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部局 |
歯学系
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