ID | 114185 |
著者 | |
キーワード | adenylate kinase
AK4
mitochondria
protein expression
immunohistochemistry
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資料タイプ |
学術雑誌論文
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抄録 | Adenylate kinase (AK) is a key enzyme in the high-energy phosphoryl transfer reaction in living cells. Of its isoforms, AK4 has a similar sequence and subcellular localization to that of AK3 in the mitochondrial matrix. However, unlike AK3, AK4 lacks the guanosine triphosphate: adenosine monophosphate phosphotransferase activity. To elucidate the physiological role of AK4, we explored the protein localization of AK4 in various mouse tissues by immunohistochemical analysis. AK4 protein was detected in the kidney, liver, brain, heart, stomach, intestine, and gonads but not in the lung and spleen. Interestingly, cell-type specific expression was evident in the brain, gastrointestinal tract, and gonads. In the cerebellum, AK4 was detected in granular cells but not in Purkinje cell bodies. In the gastrointestinal tract, AK4 was highly expressed in epithelia. In the ovary, AK4 was detected in oocytes and corpora lutea. In the testis, AK4 was detected in spermatocytes but not in spermatogonia. Our findings demonstrate that AK4 localizes uniquely in a cell-type and tissue-specific manner in mouse tissues.
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掲載誌名 |
Acta Histochemica et Cytochemica
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ISSN | 00445991
13475800
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cat書誌ID | AA00508022
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出版者 | The Japan Society of Histochemistry and Cytochemistry
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巻 | 42
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号 | 2
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開始ページ | 55
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終了ページ | 64
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発行日 | 2009
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EDB ID | |
出版社版DOI | |
出版社版URL | |
フルテキストファイル | |
言語 |
eng
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著者版フラグ |
出版社版
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部局 |
歯学系
病院
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