| ID | 113298 |
| 著者 |
Hirata, Yohko
Tokushima University
保坂, 利男
Tokushima University
Le, Chung T.K.
Tokushima University
Jambaldorj, Bayasgalan
Tokushima University
Teshigawara, Kiyoshi
Tokushima University
|
| キーワード | Vimentin
GLUT4
IRAP
|
| 資料タイプ |
学術雑誌論文
|
| 抄録 | Insulin-responsive aminopeptidase (IRAP) and GLUT4 are two major cargo proteins of GLUT4 storage vesicles (GSVs) that are translocated from a postendosomal storage compartment to the plasma membrane (PM) in response to insulin. The cytoplasmic region of IRAP is reportedly involved in retention of GSVs. In this study, vimentin was identified using the cytoplasmic domain of IRAP as bait. The validity of this interaction was confirmed by pull-down assays and immunoprecipitation in 3T3-L1 adipocytes. In addition, it was shown that GLUT4 translocation to the PM by insulin was decreased in vimentin-depleted adipocytes, presumably due to dispersing GSVs away from the cytoskeleton. These findings suggest that the IRAP binding protein, vimentin, plays an important role in retention of GSVs.
|
| 掲載誌名 |
Biochemical and Biophysical Research Communications
|
| ISSN | 0006291X
|
| cat書誌ID | AA00564395
|
| 出版者 | Elsevier
|
| 巻 | 405
|
| 号 | 1
|
| 開始ページ | 96
|
| 終了ページ | 101
|
| 発行日 | 2011-01-07
|
| 備考 | 本論文は，著者Yohko Hirataの学位論文として提出され，学位審査・授与の対象となっている。
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| EDB ID | |
| 出版社版DOI | |
| 出版社版URL | |
| フルテキストファイル | |
| 言語 |
eng
|
| 著者版フラグ |
出版社版
|
| 部局 |
歯学系
医学系
|
