ID | 114099 |
著者 |
Nishimori, Daichi
Tokushima University
Iiyama, Masamitsu
Nomura Micro Science
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キーワード | Track-etched membrane filter
Coulometric detection
Microbore/capillary liquid chromatography
Electrochemical detector
Dual-electrode system
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資料タイプ |
学術雑誌論文
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抄録 | The electrochemical flow cell containing track-etched microporous membrane electrodes was applied to a dual-electrode coulometric detector for microbore/capillary HPLC with a small injection volume and low eluent flow rate. The proposed flow cell with a 0.1-mm diameter inlet channel gave a detection volume of 0.08 nL per electrode, which was determined by the eluent flow through the electrode. For the dual-electrode detector, the calculated volume was 0.24 nL. The efficiency of electrooxidation of L-ascorbic acid increased as the flow rate decreased and was close to 100% when the flow rate was below 50 µL min−1, which is a common flow rate in microbore or capillary liquid chromatography. Catecholamines, such as noradrenaline, adrenaline, and dopamine, were detected by total conversion with two-electron oxidation in the potential range from 0.8 to 1.0 V vs. Ag/AgCl after separation with a microbore column. These peaks were accompanied by corresponding cathodic peaks derived from quasi-stable electrooxidation products of the catecholamines. The detection limits of noradrenaline, adrenaline, and dopamine were 0.1, 0.1, and 0.2 μM, respectively. The RSD values for five replicate measurements of 5.0 μM of these compounds were 0.9%, 0.7%, and 1.5%, respectively. Coulometric detection was also demonstrated by determination of catecholamines in pharmaceuticals.
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掲載誌名 |
Analytica Chimica Acta
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ISSN | 00032670
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cat書誌ID | AA00524834
AA11523130
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出版者 | Elsevier
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巻 | 1102
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開始ページ | 46
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終了ページ | 52
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発行日 | 2019-12-18
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権利情報 | © 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
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EDB ID | |
出版社版DOI | |
出版社版URL | |
フルテキストファイル | |
言語 |
eng
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著者版フラグ |
著者版
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部局 |
理工学系
技術支援部
薬学系
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