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ID 117825
著者
Ali, Hanif Tokushima University
Kobayashi, Miyu Tokushima University
Morito, Katsuya Tokushima University
Hasi, Rumana Yesmin Tokushima University
Sango, Kazunori Tokyo Metropolitan Institute of Medical Science
キーワード
Peroxisome disease
Very long-chain fatty acids
Peroxisome-deficient cells
Apoptosis
資料タイプ
学術雑誌論文
抄録
One of the major functions of peroxisomes in mammals is oxidation of very long-chain fatty acids (VLCFAs). Genetic defects in peroxisomal β-oxidation result in the accumulation of VLCFAs and lead to a variety of health problems, such as demyelination of nervous tissues. However, the mechanisms by which VLCFAs cause tissue degeneration have not been fully elucidated. Recently, we found that the addition of small amounts of isopropanol can enhance the solubility of saturated VLCFAs in an aqueous medium. In this study, we characterized the biological effect of extracellular VLCFAs in peroxisome-deficient Chinese hamster ovary (CHO) cells, neural crest-derived pheochromocytoma cells (PC12), and immortalized adult Fischer rat Schwann cells (IFRS1) using this solubilizing technique. C20:0 FA was the most toxic of the C16–C26 FAs tested in all cells. The basis of the toxicity of C20:0 FA was apoptosis and was observed at 5 μM and 30 μM in peroxisome-deficient and wild-type CHO cells, respectively. The sensitivity of wild-type CHO cells to cytotoxic C20:0 FA was enhanced in the presence of a peroxisomal β-oxidation inhibitor. Further, a positive correlation was evident between cell toxicity and the extent of intracellular accumulation of toxic FA. These results suggest that peroxisomes are pivotal in the detoxification of apoptotic VLCFAs by preventing their accumulation.
掲載誌名
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids
ISSN
13881981
cat書誌ID
AA11522965
AA11300409
出版者
Elsevier
1868
2
開始ページ
159259
発行日
2022-11-29
権利情報
© 2022. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
著者版
部局
生物資源系
薬学系