ID 111019
著者
Hoida, Ali Badr Tokushima University
Takahashi, Keiko Tokushima University
キーワード
Shiga toxin
seaweed
Ulva linza Linnaeus
rhamnan sulfate
資料タイプ
学術雑誌論文
抄録
We screened edible seaweeds in the ability to adsorb Shiga toxin (Stx) by an equilibrated dialysis method. Although water insoluble fractions of fourteen dry seaweeds did not adsorb Stx, most water soluble fractions were found to adsorb it to one degree or another. Among the seaweed tested, the extract of the Ulva linza Linnaeus [Enteromorpha linza (Linnaeus) J. Agardh] was found to well adsorb both Stx1 and Stx2. We purified the Stx-adsorbing substance from the U. linza extract by DEAE-Toyopearl column chromatography and gel filtration with HiPrep 16/60 Sephacryl S-300 HR column. The purified substance showed an average molecular mass of about 800 kDa by polyacrylamide gel electrophoresis. Analyses of its components indicated that the substance was a highly rhamnose-containing polysaccharide with sulfate esters of 18%. Apparent dissociation constants (Kd) of the polysaccharide to Stx1 and Stx2 were calculated to be 1.9 and 3.5 μM, respectively. To our knowledge, this is the first report on Stx-adsorbing dietary fibers.
掲載誌名
European Food Research and Technology
ISSN
14382377
14382385
cat書誌ID
AA11622369
出版者
Springer
243
12
開始ページ
2147
終了ページ
2153
発行日
2017-05-24
備考
This is a post-peer-review, pre-copyedit version of an article published in European Food Research and Technology. The final authenticated version is available online at: http://doi.org/10.1007/s00217-017-2915-1
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
著者版
部局
生物資源系