アクセス数 : ?
ダウンロード数 : ?
ID 25690
著者
後藤田, 貴子 Department of Legal Medicine, The University of Tokushima School of Medicine
久保, 真一 Department of Legal Medicine, The University of Tokushima School of Medicine
北村, 修 Department of Legal Medicine, The University of Tokushima School of Medicine
徳永, 逸夫 Department of Legal Medicine, The University of Tokushima School of Medicine
江口, 安希子 Department of Legal Medicine, The University of Tokushima School of Medicine
折原, 義行 Department of Legal Medicine, Nagasaki University School of Medicine
津田, 亮一 Department of Legal Medicine, Nagasaki University School of Medicine
池松, 和哉 Department of Legal Medicine, Nagasaki University School of Medicine
ナカソノ, イチロウ Department of Legal Medicine, Nagasaki University School of Medicine
キーワード
neuropathology
hypoglossal nucleus
asphyxia
neuronal damage
immunohistochemistry
資料タイプ
学術雑誌論文
抄録
The brain stem hypoglossal nucleus (HN) is the center of nerves innervating the upper respiratory tract and is related to control of mastication, deglutition, speech and respiration. To elucidate the relationship between asphyxia and the HN, we investigated the change of hypoglossal neurons in cases of hanging, strangulation, smothering, choking, drowning and respiratory failure. Using immunohistochemical techniques, we observed the brain stem HN with antibodies against microtubule-associated protein2(MAP2), muscarinic acetylcholine receptor (mAChR), c-fos gene product (c-Fos) and 72kD heat-shock protein (HSP70). MAP2, a cytoskeletal protein of the neuron, is a marker of neuronal damage. Muscarinic AChR was used as a marker of neuronal membrane and ACh signaling. We employed both HSP70 and c-Fos as markers of stress- or damage-related events. We measured the percentage of immunopositive neurons in total neurons of HN. Drowning produced higher expression of HSP70 and c-Fos than other causes of asphyxia, suggesting that drowning induces more severe damage in HN neurons. Furthermore, it was suspected that neuronal changes in drowning might relate to functions of the HN. These observations indicate that immunohistochemical examination of the brain stem HN could provide useful information for determining the cause of asphyxia.
掲載誌名
The journal of medical investigation : JMI
ISSN
13431420
cat書誌ID
AA11166929
47
1-2
開始ページ
76
終了ページ
79
並び順
76
発行日
2000
備考
EDB ID
フルテキストファイル
言語
eng