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ID 25704
著者
山野, 修司 Center for Maternity and Perinatal Care, The University of Tokushima School of Medicine
ナカガワ, コウジ Department of Obstetrics and Gynecology, The University of Tokushima School of Medicine
ナカサカ, ヒサヨ Department of Obstetrics and Gynecology, The University of Tokushima School of Medicine
青野, 敏博 Department of Obstetrics and Gynecology, The University of Tokushima School of Medicine
キーワード
ertilization
oocyte activation
parthenogenone
MPF
MAPK
資料タイプ
学術雑誌論文
抄録
Recent reports have suggested that when unfertilized oocytes with a sperma-tozoon after intracytoplasmic sperm injection (ICSI) are properly activated, the activated oocytes develop normaly similar to fertilized oocytes. However, human oocytes do not easily respond to universal activators of mammalian oocytes like ethanol or calcium ionophore A23187, which induce a calcium transient in ooplasm. Puromycin activates human oocytes at a rate of 90%, but more than two thirds of the parthenogenones possess 2 haploid pronuclei or 1 diploid pronucleus without extrusion of the second polar body. Therefore, the activation method which produces one pronucleus with extrusion of the second polar body in oocytes without a spermatozoon is necessary for producing embryos with normal karyotypes. Recently, we found the oocyte activation method which produced parthenogenones displaying one pronucleus with extrusion of the second polar body. Using our method (a combination of calcium ionophore A23187 and puromycin), the activation rate was approximately 90% and the proportion of parthenogenones dis-playing one pronucleus with extrusion of the second polar body was approximately 80% in human aged and mouse young oocytes. When human unfertilized oocytes following ICSI were activated by this method, two pronuclei were formed with extrusion of the second polar body in 30% of the oocytes. Four cleaved parthenogenones (or embryos) showed normal karyotypes. However, the cytotoxic, teratogenetic and mutagenetic activity of Ca ionophore and puromycin should be approved prior to the clinical adaptation of the method.
掲載誌名
The journal of medical investigation : JMI
ISSN
13431420
cat書誌ID
AA11166929
47
1-2
開始ページ
1
終了ページ
8
並び順
1
発行日
2000
備考
EDB ID
フルテキストファイル
言語
eng