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ID 118797
タイトル別表記
Urinary adiponectin as a biomarker for DKD
著者
Kawano, Tomoharu Tokushima Bunri University
Aiba, Motohiro Tokushima Bunri University
Hashida, Seiichi Ehime University
Colpitts, Tracey Sysmex R&D Center Americas, Inc.
Watanabe, Toshihiro Sysmex R&D Center Americas, Inc.
キーワード
Immune complex transfer enzyme immunoassay
Ultrasensitive immunoassay
Urinary adiponectin
Kidney disease
Biomarker
資料タイプ
学術雑誌論文
抄録
We previously developed two immune complex transfer enzyme immunoassays (ICT-EIA) to measure total adiponectin (T-AN) and high molecular weight adiponectin (H-AN) in urine and have verified their usefulness as biomarkers for diabetic kidney disease. In this study, we developed T-AN and H-AN assays using the sandwich EIA (Sand-EIA). The reactivities of Sand-EIAs were compared with ICT-EIAs by measuring size exclusion chromatography (SEC) fractions of urine and adiponectin standard. As a result, ICT-EIAs showed higher macromolecular specificity. We then analyzed the molecular profile of adiponectin in the urine of 5 patients with different eGFR stages by measuring SEC fractions of urine. The results showed that smaller adiponectin correlated relatively well with eGFR stage. Finally, because SEC is time-consuming, we investigated that the ratio of T-ANs by Sand-EIA and ICT-EIA could be a good indicator of the monomer adiponectin. The ratio was evaluated using 77 urine samples from patients with diabetes and showed a significant decrease at an earlier stage compared with other biomarkers. In conclusion, we demonstrated a new index to estimate monomer adiponectin in urine by using Sand-EIA and ICT-EIA, and urinary monomer adiponectin can be a good early indicator of deterioration of renal function in diabetic patients.
掲載誌名
The Journal of Medical Investigation
ISSN
13496867
13431420
cat書誌ID
AA11166929
出版者
Tokushima University Faculty of Medicine
70
3-4
開始ページ
464
終了ページ
470
並び順
464
発行日
2023-08
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
医学系
先端酵素学研究所