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ID 114964
著者
Ogura, Yoshitoshi Kyushu University
Mondal, Shakhinur Islam University of Miyazaki|Shahjalal University of Science and Technology
Islam, Md Rakibul University of Miyazaki
Mako, Toshihiro Fukuoka City Institute for Hygiene and the Environment
Katsura, Keisuke University of Miyazaki
Ooka, Tadasuke Kagoshima University
Gotoh, Yasuhiro University of Miyazaki
Murase, Kazunori University of Miyazaki
Ohnishi, Makoto National Institute of Infectious Diseases
Hayashi, Tetsuya Kyushu University
資料タイプ
学術雑誌論文
抄録
Enterohemorrhagic E. coli (EHEC) causes diarrhea and hemorrhagic colitis with life-threatening complications, such as hemolytic uremic syndrome. Their major virulence factor is Shiga toxin (Stx), which is encoded by bacteriophages. Of the two types of Stx, the production of Stx2, particularly that of Stx2a (a subtype of Stx2), is a major risk factor for severe EHEC infections, but the Stx2 production level is highly variable between strains. Here, we define four major and two minor subtypes of Stx2a-encoding phages according to their replication proteins. The subtypes are correlated with Stx2a titers produced by the host O157 strains, suggesting a critical role of the phage subtype in determining the Stx2a production level. We further show that one of the two subclades in the clade 8, a proposed hyper-virulent lineage of O157, carries the Stx2 phage subtype that confers the highest Stx2 production to the host strain. The presence of this subclade may explain the proposed high virulence potential of clade 8. These results provide novel insights into the variation in virulence among O157 strains and highlight the role of phage variation in determining the production level of the virulence factors that phages encode.
掲載誌名
Scientific Reports
ISSN
20452322
出版者
Springer Nature
5
開始ページ
16663
発行日
2015-11-16
権利情報
This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
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言語
eng
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部局
医学系