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ID 110623
タイトルヨミ
キュウキュウ ニオケル ステロイド ゴウセイ コウソ ノ キョクザイ
タイトル別表記
Localization of steroid-synthesizing enzymes in the olfactory bulb
著者
清蔭, 恵美 徳島大学医学部情報統合医学講座形態情報医学分野
樋田, 一徳 徳島大学医学部情報統合医学講座形態情報医学分野
山本, 登志子 徳島大学医学部情報統合医学講座形態情報医学分野
石村, 和敬 徳島大学医学部情報統合医学講座形態情報医学分野 徳島大学 教育研究者総覧 KAKEN研究者をさがす
キーワード
neurosteroid
olfactory bulb
enzyme
steroid
immunohistochemistry
localization
資料タイプ
その他
抄録
Neurosteroids are synthesized through mechanism at least partly independent of the peripheral steroidogenic glands, and their neurobiological actions seem to depend on the specific functions of various brain regions. However, little is known about neurosteroids function corresponding to specific structure and functions of the brain regions. Thus the present study analyzed localization of steroid-synthesizing enzymes in the rat and mouse olfactory bulb. RT-PCR and Western-blotting indicated the possible presence of almost all enzymes of steroid synthesis from cholesterol to estradiol, that is, cholesterol side chain cleavage enzyme (P450scc : cholesterol→pregnenolone), 3β-hydroxysteroid dehydrogenase/Δ5 - 4 isomerase (3β-HSD : pregnenolone→progesterone), 17α-hydroxylase/C17-C20lyase (P450c17 : progesterone→ androstenedione), 17β-hydroxysteroid dehydrogenase (17β-HSD : androstenedione→testosterone), 5α-reductase (5αR:testosterone→5α-dihydrotestosterone), and aromatase (testosterone→ estradiol). Immunohistochemistry confirmed that the 5α-reductase was mainly in glial cells with various immunoreactivity and co-localization pattern, but other enzymes were in bulbar neurons, and at least co-localized in mitral/tufted cells. In addition, so far analyzed, enzymatic activities of P450c17 (a key enzyme in sex steroid synthesis) and 17β-HSD were detected biochemically. These enzyme expression and activities were influenced under exposure of 17β-estradiol with various degrees. These findings indicated the presence of steroid-synthesizing activities, and thus of possible neurosteroid metabolism in the olfactory bulb.
掲載誌名
四国医学雑誌
ISSN
00373699
cat書誌ID
AN00102041
出版者
徳島医学会
59
4-5
開始ページ
250
終了ページ
251
並び順
250
発行日
2003-10-25
EDB ID
フルテキストファイル
言語
jpn
著者版フラグ
出版社版
部局
医学系