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ID 116451
タイトル別表記
The role of AmeloD in tooth development
著者
Chiba, Yuta National Institutes of Health|Tohoku University
He, Bing National Institutes of Health|Sichuan University
Yoshizaki, Keigo National Institutes of Health|Kyushu University
Rhodes, Craig National Institutes of Health
Ishijima, Muneaki National Institutes of Health|Juntendo University
Bleck, Christopher K. E. National Institutes of Health
Stempinski, Erin National Institutes of Health
Chu, Emily Y. National Institutes of Health
Nakamura, Takashi National Institutes of Health|Tohoku University
岩本, 勉 National Institutes of Health|Tokushima University KAKEN研究者をさがす
de Vega, Susana National Institutes of Health|Juntendo University
Saito, Kan Tohoku University
Fukumoto, Satoshi National Institutes of Health|Tohoku University
Yamada, Yoshihiko National Institutes of Health
資料タイプ
学術雑誌論文
抄録
The development of ectodermal organs, such as teeth, requires epithelial–mesenchymal interactions. Basic helix–loop–helix (bHLH) transcription factors regulate various aspects of tissue development, and we have previously identified a bHLH transcription factor, AmeloD, from a tooth germ cDNA library. Here, we provide both in vitro and in vivo evidence that AmeloD is important in tooth development. We created AmeloD-knockout (KO) mice to identify the in vivo functions of AmeloD that are critical for tooth morphogenesis. We found that AmeloD-KO mice developed enamel hypoplasia and small teeth because of increased expression of E-cadherin in inner enamel epithelial (IEE) cells, and it may cause inhibition of the cell migration. We used the CLDE dental epithelial cell line to conduct further mechanistic analyses to determine whether AmeloD overexpression in CLDE cells suppresses E-cadherin expression and promotes cell migration. Knockout of epiprofin (Epfn), another transcription factor required for tooth morphogenesis and development, and analysis of AmeloD expression and deletion revealed that AmeloD also contributed to multiple tooth formation in Epfn-KO mice by promoting the invasion of dental epithelial cells into the mesenchymal region. Thus, AmeloD appears to play an important role in tooth morphogenesis by modulating E-cadherin and dental epithelial–mesenchymal interactions. These findings provide detailed insights into the mechanism of ectodermal organ development.
掲載誌名
Journal of Biological Chemistry
ISSN
00219258
cat書誌ID
AA1202441X
出版者
American Society for Biochemistry and Molecular Biology|Elsevier
294
10
開始ページ
3406
終了ページ
3418
発行日
2018-11-30
権利情報
This is an Open Access article under the CC BY license(https://creativecommons.org/licenses/by/4.0/).
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
歯学系