β5t shapes CD8 T cells without negative selection
Frantzeskakis, Melina National Institutes of Health
Jacques, Alison National Institutes of Health
Yamashita, Fusano University of Tokushima
Yin, Da National Institutes of Health
Cam, Margaret National Institutes of Health
Kelly, Michael C. National Institutes of Health
Awasthi, Parirokh National Institutes of Health
The thymoproteasome expressed specifically in thymic cortical epithelium optimizes the generation of CD8+ T cells; however, how the thymoproteasome contributes to CD8+ T cell development is unclear. Here, we show that the thymoproteasome shapes the TCR repertoire directly in cortical thymocytes before migration to the thymic medulla. We further show that the thymoproteasome optimizes CD8+ T cell production independent of the thymic medulla; independent of additional antigen-presenting cells, including medullary thymic epithelial cells and dendritic cells; and independent of apoptosis-mediated negative selection. These results indicate that the thymoproteasome hardwires the TCR repertoire of CD8+ T cells with cortical positive selection independent of negative selection in the thymus.
Journal of Experimental Medicine
Rockefeller University Press
This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).
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