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ID 110120
タイトル別表記
過剰負荷を受けた顎関節におけるヒアルロン酸代謝の検討
Hyaluronan metabolism in overloaded TMJ
著者
篠原, 丈裕 徳島大学大学院口腔科学教育部(口腔科学専攻)
三野, 彰子 Tokushima University
犬伏, 俊博 Sanford Burnham Medical Research Institute
Yamaguchi, Y Sanford Burnham Medical Research Institute
キーワード
Hyaluronan
temporomandibular joint
osteoarthritis
資料タイプ
学位論文
抄録
The present study aimed to examine hyaluronan (HA) metabolism in relation to the onset and progression of temporomandibular joint osteoarthritis (TMJ-OA) induced by mechanical overloading. Two-month-old and 6-month-old C57BL/6N mice were divided into experimental and untreated control groups (n = 5/group). A sliding plate was attached to the maxillary incisors of the experimental mice for 10 days to overload the condylar cartilage in TMJ. In experimental group, profound cartilage degradation was detected in hematoxylin-eosin, Safranin-O-Fast Green stained sections. It was also shown that the cartilage degradation was greater in older mice in both the control and the experimental groups. The number of HABP positive cells was decreased by mechanical overloading and with age. The reduction of HA expression was correlated with the progression of cartilage degradation induced by mechanical overloading. The absolute quantification of the mRNA expression related to HA synthesis and HA degradation was also performed in each group. The mRNA expression levels of HA synthase (HAS) 2 and 3 were lower in the experimental group compared with the control group in the younger mice. In contrast, the mRNA expression levels of the HA degradation gene, HYAL2 and KIAA1199, were higher in the experimental group compared with the control group in the older mice. Thus, mechanical overload differently affected the balance of HA degradation and HA synthesis in the older and younger mice, respectively. In conclusion, mechanical overloading affects HA metabolism and it might initiate or amplify the condylar cartilage degradation.
掲載誌名
Journal of Oral Rehabilitation
ISSN
0305182X
13652842
cat書誌ID
AA00704042
AA11628445
出版者
John Wiley & Sons
43
12
開始ページ
921
終了ページ
928
発行日
2016-09-14
備考
内容要旨・審査要旨・論文本文の公開:
内容要旨:LID201704281007.pdf
審査要旨:LID201704281008.pdf
論文本文:k3073_fulltext.pdf
著者の申請により要約(2017-04-28公開)に替えて論文全文を公開(2019-06-13)
本論文は, 著者T. Shinoharaの学位論文として提出され, 学位審査・授与の対象となっている。
権利情報
This is the peer reviewed version of the following article: Shinohara, T. , Izawa, T. , Mino‐Oka, A. , Mori, H. , Iwasa, A. , Inubushi, T. , Yamaguchi, Y. and Tanaka, E. (2016), Hyaluronan metabolism in overloaded temporomandibular joint. J Oral Rehabil, 43: 921-928. doi:10.1111/joor.12443, which has been published in final form at https://doi.org/10.1111/joor.12443. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第3073号
学位記番号
甲口第416号
学位授与年月日
2017-03-23
学位名
博士(歯学)
学位授与機関
徳島大学
部局
歯学系