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ID 110153
著者
ニシタニ, チカコ NARO Institute of Fruit Tree and Tea Science
ヒライ, ナルミ NARO Institute of Fruit Tree and Tea Science
コモリ, サダオ Faculty of Agriculture, Iwate University
ワダ, マサト NARO Institute of Fruit Tree and Tea Science
オカダ, カズマ NARO Institute of Fruit Tree and Tea Science
刑部, 敬史 Faculty of Bioscience and Bioindustry, Tokushima University 徳島大学 教育研究者総覧 KAKEN研究者をさがす
ヤマモト, トシヤ NARO Institute of Fruit Tree and Tea Science
刑部, 祐里子 Faculty of Bioscience and Bioindustry, Tokushima University 徳島大学 教育研究者総覧 KAKEN研究者をさがす
資料タイプ
学術雑誌論文
抄録
Genome editing is a powerful technique for genome modification in molecular research and crop breeding, and has the great advantage of imparting novel desired traits to genetic resources. However, the genome editing of fruit tree plantlets remains to be established. In this study, we describe induction of a targeted gene mutation in the endogenous apple phytoene desaturase (PDS) gene using the CRISPR/Cas9 system. Four guide RNAs (gRNAs) were designed and stably transformed with Cas9 separately in apple. Clear and partial albino phenotypes were observed in 31.8% of regenerated plantlets for one gRNA, and bi-allelic mutations in apple PDS were confirmed by DNA sequencing. In addition, an 18-bp gRNA also induced a targeted mutation. These CRIPSR/Cas9 induced-mutations in the apple genome suggest activation of the NHEJ pathway, but with some involvement also of the HR pathway. Our results demonstrate that genome editing can be practically applied to modify the apple genome.
掲載誌名
Scientific Reports
ISSN
20452322
6
開始ページ
31481
並び順
31481
発行日
2016-08-17
備考
Copyright ©2016 Authors.This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
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フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
生物資源系