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ID 110164
著者
ウエタ, リサ Graduate School of Advanced Technology and Science, Tokushima University
アベ, チヒロ Graduate School of Advanced Technology and Science, Tokushima University
ワタナベ, タカヒロ Center for Collaboration among Agriculture, Industry, and Commerce, Tokushima University
スガノ, シゲオ Center for Collaboration among Agriculture, Industry, and Commerce, Tokushima University
イシハラ, リョウスケ Graduate School of Advanced Technology and Science, Tokushima University
エズラ, ヒロシ Graduate School of Life and Environmental Sciences, University of Tsukuba
刑部, 祐里子 Faculty of Bioscience and Bioindustry, Tokushima University 徳島大学 教育研究者総覧 KAKEN研究者をさがす
刑部, 敬史 Faculty of Bioscience and Bioindustry, Tokushima University 徳島大学 教育研究者総覧 KAKEN研究者をさがす
資料タイプ
学術雑誌論文
抄録
Parthenocarpy in horticultural crop plants is an important trait with agricultural value for various industrial purposes as well as direct eating quality. Here, we demonstrate a breeding strategy to generate parthenocarpic tomato plants using the CRISPR/Cas9 system. We optimized the CRISPR/Cas9 system to introduce somatic mutations effectively into SlIAA9—a key gene controlling parthenocarpy— with mutation rates of up to 100% in the T0 generation. Furthermore, analysis of off-target mutations using deep sequencing indicated that our customized gRNAs induced no additional mutations in the host genome. Regenerated mutants exhibited morphological changes in leaf shape and seedless fruit—a characteristic of parthenocarpic tomato. And the segregated next generation (T1) also showed a severe phenotype associated with the homozygous mutated genome. The system developed here could be applied to produce parthenocarpic tomato in a wide variety of cultivars, as well as other major horticultural crops, using this precise and rapid breeding technique.
掲載誌名
Scientific Reports
ISSN
20452322
7
開始ページ
507
並び順
507
発行日
2017-03-30
備考
© The Author(s) 2017 This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
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出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
生物資源系