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ID 111281
タイトル別表記
フクロネズミ腎近位尿細管細胞におけるナトリウム依存性リン酸トランスポーターNaPi-IIcと細胞内vacuole形成の関連
NaPi-IIc induces vacuole formation in OK cells
著者
塩﨑, 雄治 徳島大学大学院栄養生命科学教育部(人間栄養科学専攻) 徳島大学 教育研究者総覧
Ohnishi, Saori University of Tokushima
Ohi, Akiko University of Tokushima
Ito, Mikiko University of Hyogo
Kido, Shinsuke Kinki University
キーワード
proximal tubule
SLC34A3
opossum kidney cells
proliferation
vacuole
資料タイプ
学位論文
抄録
NaPi-IIc/SLC34A3 is a sodium-dependent inorganic phosphate (Pi) transporter in the renal proximal tubules and its mutations cause hereditary hypophosphatemic rickets with hypercalciuria (HHRH). In the present study, we created a specific antibody for opossum SLC34A3, NaPi-IIc (oNaPi-IIc), and analyzed its localization and regulation in opossum kidney cells (a tissue culture model of proximal tubular cells). Immunoreactive oNaPi-IIc protein levels increased during the proliferative phase and decreased during differentiation. Moreover, stimulating cell growth upregulated oNaPi-IIc protein levels, whereas suppressing cell proliferation downregulated oNaPi-IIc protein levels. Immunocytochemistry revealed that endogenous and exogenous oNaPi-IIc proteins localized at the protrusion of the plasma membrane, which is a phosphatidylinositol 4,5-bisphosphate (PIP2) rich-membrane, and at the intracellular vacuolar membrane. Exogenous NaPi-IIc also induced cellular vacuoles and localized in the plasma membrane. The ability to form vacuoles is specific to electroneutral NaPi-IIc, and not electrogenic NaPi-IIa or NaPi-IIb. In addition, mutations of NaPi-IIc (S138F and R468W) in HHRH did not cause cellular PIP2-rich vacuoles. In conclusion, our data anticipate that NaPi-IIc may regulate PIP2 production at the plasma membrane and cellular vesicle formation.
掲載誌名
The Journal of Medical Investigation
ISSN
13496867
13431420
cat書誌ID
AA11166929
AA12022913
出版者
Faculty of Medicine Tokushima University
62
3-4
開始ページ
209
終了ページ
218
並び順
209
発行日
2015-08
備考
内容要旨・審査要旨・論文本文の公開:
内容要旨・審査要旨 : LID201508051001.pdf
論文本文 : LID201509301001.pdf
本論文は,著者Yuji Shiozakiの学位論文として提出され,学位審査・授与の対象となっている。
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第2867号
学位記番号
甲栄第230号
学位授与年月日
2015-07-23
学位名
博士(栄養学)
学位授与機関
徳島大学
部局
医学系