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タイトル別表記
Giantin Affects Golgi Stack Connection
著者
Satoh, Ayano Okayama University
Hayashi-Nishino, Mitsuko Osaka University
Shakuno, Takuto Okayama University
Masuda, Junko Okayama University
Koreishi, Mayuko Okayama University
Murakami, Runa Okayama University
Nakamura, Yoshimasa Okayama University
中村, 俊之 Okayama University
叶, 奈緒美 Okayama University|Tokushima University 徳島大学 教育研究者総覧
Honjo, Yasuko Hiroshima University
Malsam, Joerg Heidelberg University
Yu, Sidney The Chinese University of Hong Kong
Nishino, Kunihiko Osaka University
キーワード
Golgi
golgins
glycosylation
endoplasmic reticulum
electron tomography
資料タイプ
学術雑誌論文
抄録
Golgins are a family of Golgi-localized long coiled-coil proteins. The major golgin function is thought to be the tethering of vesicles, membranes, and cytoskeletal elements to the Golgi. We previously showed that knockdown of one of the longest golgins, Giantin, altered the glycosylation patterns of cell surfaces and the kinetics of cargo transport, suggesting that Giantin maintains correct glycosylation through slowing down transport within the Golgi. Giantin knockdown also altered the sizes and numbers of mini Golgi stacks generated by microtubule de-polymerization, suggesting that it maintains the independence of individual Golgi stacks. Therefore, it is presumed that Golgi stacks lose their independence following Giantin knockdown, allowing easier and possibly increased transport among stacks and abnormal glycosylation. To gain structural insights into the independence of Golgi stacks, we herein performed electron tomography and 3D modeling of Golgi stacks in Giantin knockdown cells. Compared with control cells, Giantin-knockdown cells had fewer and smaller fenestrae within each cisterna. This was supported by data showing that the diffusion rate of Golgi membrane proteins is faster in Giantin-knockdown Golgi, indicating that Giantin knockdown structurally and functionally increases connectivity among Golgi cisternae and stacks. This increased connectivity suggests that contrary to the cis-golgin tether model, Giantin instead inhibits the tether and fusion of nearby Golgi cisternae and stacks, resulting in transport difficulties between stacks that may enable the correct glycosylation of proteins and lipids passing through the Golgi.
掲載誌名
Frontiers in Cell and Developmental Biology
ISSN
2296634X
出版者
Frontiers Media S.A.
7
開始ページ
160
発行日
2019-08-27
権利情報
Copyright © 2019 Satoh, Hayashi-Nishino, Shakuno, Masuda, Koreishi, Murakami, Nakamura, Nakamura, Abe-Kanoh, Honjo, Malsam, Yu and Nishino. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). (https://creativecommons.org/licenses/by/4.0/) The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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言語
eng
著者版フラグ
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部局
医学系