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ID 114475
著者
Yanagimoto, Yoshiyuki PROVIGATE
Saito, Akiko The University of Tokyo
Sakata, Toshiya The University of Tokyo
キーワード
biomimetic material
poly(catecholamine)
pH-sensitive biosensor
voltage follower circuit
cell sensing
資料タイプ
学術雑誌論文
抄録
Surface-coated poly(catecholamine) (pCA) films have attracted attention as biomaterial interfaces owing to their biocompatible and physicochemical characteristics. In this paper, we report that pCA-film-coated electrodes are useful for potentiometric biosensing devices. Four different types of pCA film—L-dopa, dopamine, norepinephrine, and epinephrine—with thicknesses in the range of 7–27 nm were electropolymerized by oxidation on Au electrodes by using cyclic voltammetry. By using the pCA-film electrodes, the pH responsivities were found to be 39.3 to 47.7 mV/pH within the pH range of 1.68 to 10.01 on the basis of the equilibrium reaction with hydrogen ions and the functional groups of the pCAs. The pCA films suppressed nonspecific signals generated by other ions (Na+, K+, Ca2+) and proteins such as albumin. Thus, the pCA-film electrodes can be used in pH-sensitive and pH-selective biosensors. HeLa cells were cultivated on the surface of the pCA-film electrodes to monitor cellular activities. The surface potential of the pCA-film electrodes changed markedly because of cellular activity; therefore, the change in the hydrogen ion concentration around the cell/pCA-film interface could be monitored in real time. This was caused by carbon dioxide or lactic acid that is generated by cellular respiration and dissolves in the culture medium, resulting in the change of hydrogen concentration. pCA-film electrodes are suitable for use in biocompatible and pH-responsive biosensors, enabling the more selective detection of biological phenomena.
掲載誌名
ACS Sensors
ISSN
23793694
cat書誌ID
AA12774646
出版者
ACS Publications
3
2
開始ページ
476
終了ページ
483
発行日
2018-01-23
権利情報
This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Sensors, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acssensors.7b00897.
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
著者版
部局
ポストLEDフォトニクス研究所