アクセス数 : ?
ダウンロード数 : ?
ID 116060
タイトル別表記
変形性顎関節症の進展におけるp21の免疫調節作用
著者
フゥレル オチル, ツェンドスレン 徳島大学大学院口腔科学教育部(口腔科学専攻)
キーワード
p21
chondrocytes
temporomandibular joint
osteoarthritis
metalloprotease
chondrocyte
資料タイプ
学位論文
抄録
Objective: We aimed to identify the immunoregulatory role of the cyclin-dependent kinase inhibitor p21 in the homeostasis of mandibular condylar cartilage affected by mechanical stress.
Materials and Methods: Ten C57BL/6 wild-type (WT) and ten p21–/– mice aged 8 weeks were divided into the untreated and treated groups. In the treated groups, mechanical stress was applied to the temporomandibular joint (TMJ) through forced mouth opening for 3 h/day for 7 days. The dissected TMJs were assessed using micro-CT, histology, and immunohistochemistry.
Results: Treated p21–/– condyles with mechanical stress revealed more severe subchondral bone destruction, with thinner cartilage layers and smaller proteoglycan area relative to treated WT condyles; untreated WT and p21–/– condyles had smoother surfaces. Immunohistochemistry revealed significant increases in the numbers of Caspase-3, interleukin-1β, matrix metalloprotease (MMP)-9, and MMP-13 positive cells, and few aggrecan positive cells, in treated p21–/– than in treated WT samples. Moreover, the number of TUNEL positive cells markedly increased in p21–/– condyles affected by mechanical stress.
Conclusion: Our findings indicate that p21 in chondrocytes contributes to regulate matrix synthesis via the control of aggrecan and MMP-13 expression under mechanical stress. Thus, p21 might regulate the pathogenesis of osteoarthritis in the TMJ.
掲載誌名
Clinical and Experimental Dental Research
ISSN
20574347
出版者
Wiley
7
3
開始ページ
313
終了ページ
322
発行日
2021-02-10
備考
内容要旨・審査要旨・論文本文の公開
本論文は,著者Tsendsuren Khurel-Ochirの学位論文として提出され,学位審査・授与の対象となっている。
権利情報
This is an open access article under the terms of the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/4.0/ ), which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第3493号
学位記番号
甲口第471号
学位授与年月日
2021-03-23
学位名
博士(歯学)
学位授与機関
徳島大学
部局
歯学系