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ID 117618
タイトル別表記
同系移植された脂肪由来幹細胞から分化したインスリン産生細胞は、NODマウスの自己免疫反応により遅発性の損傷を受ける
著者
徳田, 和憲 徳島大学大学院医学研究科(医学専攻)
Miyazaki, Katsuki Tokushima University
Okikawa, Shohei Tokushima University
キーワード
insulin producing cell (IPC)
65-kDa isoform of glutamic acid decarboxylase (GAD65)
programmed cell death ligand 1 (PD-L1)
type 1 diabetes mellitus
non-obese diabetic mouse
資料タイプ
学位論文
抄録
Insulin-producing cells (IPCs) generated by our established protocol have reached the non-clinical ‘proof of concept’ stage. Our strategy for their clinical application is the autotransplantation of IPCs into patients with type 1 diabetes mellitus (T1DM). In this context, the autoimmunity that characterized T1DM is important, rather than allorejection. We aimed to determine how these IPCs respond to T1DM autoimmunity. IPCs were generated from the subcutaneous fat tissue of non-obese diabetic (NOD) mice using our protocol. IPCs derived from NOD mice were transplanted under the kidney capsules of NOD mice at the onset of diabetes and the subsequent changes in blood glucose concentration were characterized. Blood glucose decreased within 30 days of transplantation, but increased again after 40–60 days in three of four recipient NOD mice. In tissue samples, the numbers of CD4+ and CD8+ T cells were significantly higher 60 days after transplantation than 30 days after transplantation. In conclusion, IPCs significantly ameliorate the diabetes of mice in the short term, but are damaged by autoimmunity in the longer term, as evidenced by local T cells accumulation. This study provides new insights into potential stem cell therapies for T1DM.
掲載誌名
Scientific Reports
ISSN
20452322
出版者
Springer Nature
12
開始ページ
5852
発行日
2022-04-07
備考
内容要旨・審査要旨・論文本文の公開
本論文は,著者Kazunori Tokudaの学位論文として提出され,学位審査・授与の対象となっている。
権利情報
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第3663号
学位記番号
甲医第1545号
学位授与年月日
2022-09-22
学位名
博士(医学)
学位授与機関
徳島大学
部局
病院
医学系