振幅変調多重化フロー分析法

In this tutorial review, the principle of amplitude modulated multiplexed flow analysis, a continuous flow analysis coupled with fast Fourier transform, FFT, is described. The flow rates of sample solutions are respectively varied at different frequencies. The solutions are merged, and then air bubbles are introduced to the merged stream so as to limit the axial dispersion of analytes that causes amplitude damping. The stream is led to a detector with no physical deaeration. Air signals are removed by signal processing, and the resulting liquid signals are analyzed by FFT. The analytes in the samples are respectively determined from the amplitudes of the corresponding wave components. Temporal profile of amplitudes can be obtained in real-time by moving the window for FFT with time.