アクセス数 : ?
ダウンロード数 : ?
ID 114741
著者
Teramoto, Machiko Kyoto Sangyo University
Sugawara, Ryo Kyoto Sangyo University
Minegishi, Katsura Nagoya University
Uchikawa, Masanori Osaka University
Kuroiwa, Atsushi Nagoya University
Ishii, Yasuo Kyoto Sangyo University|Tokyo Women’s Medical University
Kondoh, Hisato Kyoto Sangyo University
キーワード
Anterior foregut
Conditional knockout
Epithelial-mesenchymal interaction
Esophagus
Respiratory organ
SOX2
資料タイプ
学術雑誌論文
抄録
In the anterior foregut (AFG) of mouse embryos, the transcription factor SOX2 is expressed in the epithelia of the esophagus and proximal branches of respiratory organs comprising the trachea and bronchi, whereas NKX2.1 is expressed only in the epithelia of respiratory organs. Previous studies using hypomorphic Sox2 alleles have indicated that reduced SOX2 expression causes the esophageal epithelium to display some respiratory organ characteristics. In the present study, we produced mouse embryos with AFG-specific SOX2 deficiency. In the absence of SOX2 expression, a single NKX2.1-expressing epithelial tube connected the pharynx and the stomach, and a pair of bronchi developed in the middle of the tube. Expression patterns of NKX2.1 and SOX9 revealed that the anterior and posterior halves of SOX2-deficient AFG epithelial tubes assumed the characteristics of the trachea and bronchus, respectively. In addition, we found that mesenchymal tissues surrounding the SOX2-deficient NKX2.1-expressing epithelial tube changed to those surrounding the trachea and bronchi in the anterior and posterior halves, as indicated by the arrangement of smooth muscle cells and SOX9-expressing cells and by the expression of Wnt4 (esophagus specific), Tbx4 (respiratory organ specific), and Hoxb6 (distal bronchus specific). The impact of mesenchyme-derived signaling on the early stage of AFG epithelial specification has been indicated. Our study demonstrated an opposite trend where epithelial tissue specification causes concordant changes in mesenchymal tissues, indicating a reciprocity of epithelial-mesenchymal interactions.
掲載誌名
Biology Open
ISSN
20466390
出版者
The Company of Biologists
9
2
開始ページ
bio048728
発行日
2020-02-07
権利情報
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
先端酵素学研究所