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ナローバンドUVBがアレルギー性鼻炎モデルラットの鼻症状とヒスタミンH1受容体mRNAの発現亢進へ与える影響
Narrow-band UVB suppresses nasal symptom and H1R mRNA
著者
神村, 盛一郎 徳島大学大学院医科学教育部(医学専攻) 徳島大学 教育研究者総覧
Okamoto, Kentaro Tokushima University
Sanada, Nanae Tokushima University
Okajima, Natsuki Tokushima University
Wakugawa, Tomoharu Tokushima University
キーワード
Allergic rhinitis
apoptosis
histamine H1 receptor
narrow-band ultraviolet B
phototherapy
資料タイプ
学位論文
抄録
Background: Phototherapy with narrow-band ultraviolet B (narrow-band UVB) is clinically effective treatment for atopic dermatitis. In the present study, we examined the effects of intranasal irradiation with narrow-band UVB on nasal symptom, upregulation of histamine H1 receptor (H1R) gene expression and induction of DNA damage in the nasal mucosa of allergic rhinitis (AR) model rat.
Methods: AR model rats were intranasally irradiated with 310 nm of narrow-band UVB. Nasal mucosal levels of H1R mRNA were measured using real-time quantitative reverse transcriptase (RT)-PCR. DNA damage was evaluated using cyclobutane pyrimidine dimer (CPD) immunostaining.
Results: In toluene 2, 4-diisocyanate (TDI)-sensitized rats, TDI provoked sneezes and H1R gene expression in the nasal mucosa. Intranasal pre-irradiation with 310 nm narrow-band UVB at doses of 600 and 1400, but not 200 mJ/cm2 significantly inhibited the number of sneezes and upregulation of H1R gene expression provoked by TDI. CPD-positive cells appeared in the nasal mucosa after intranasal narrow-band UVB irradiation at a dose of 1400, but not 200 and 600 mJ/cm2. The suppression of TDI-provoked sneezes and upregulation of H1R gene expression lasted 24 h, but not 48 h, after narrow-band UVB irradiation with a dose of 600 mJ/cm2.
Conclusions: Intranasal pre-irradiation with narrow-band UVB dose-dependently inhibited sneezes and upregulation of H1R gene expression of the nasal mucosa in AR model rats, suggesting that the inhibition of nasal upregulation of H1R gene expression suppressed nasal symptom. The suppression after narrow-band UVB irradiation at a dose of 600 mJ/cm2 was reversible without induction of DNA damage. These findings indicated that low-dose narrow-band UVB phototherapy could be effectively and safely used for AR treatment in a clinical setting.
掲載誌名
Laryngoscope Investigative Otolaryngology
ISSN
23788038
出版者
John Wiley & Sons, Inc.|The Triological Society
6
1
開始ページ
34
終了ページ
41
発行日
2021-01-08
備考
内容要旨・審査要旨・論文本文の公開
本論文は,著者Seiichiro Kamimuraの学位論文として提出され,学位審査・授与の対象となっている。
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第3496号
学位記番号
甲医第1482号
学位授与年月日
2021-03-23
学位名
博士(医学)
学位授与機関
徳島大学
部局
病院
薬学系
医学系