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ID 113340
タイトル別表記
Epigallocatechin-3-gallateは大腸癌細胞株においてNrf2とオートファジーを介して放射線感受性を増強する
EGCG Enhances Radiation Sensitivity in Colorectal Cancer Cells
著者
エンクバト, トゥメンジン 徳島大学大学院医科学教育部(医学専攻)
キーワード
Epigallocatechin-3-gallate
Radiation
Colorectal cancer
Nuclear factor (erythroid-derived 2)-like 2
Autophagy
資料タイプ
学位論文
抄録
Background/Aim: Epigallocatechin-3-gallate (EGCG) is a major polyphenolic component of green tea. EGCG plays a potential role in radio-sensitizing cancer cells. The combined effect of EGCG and radiation was investigated in a colorectal cancer cell line, focusing on nuclear factor (erythroid-derived 2)-like 2 (Nrf2) autophagy signalling. Materials and Methods: HCT-116 cells were treated with 12.5 μM EGCG for different periods of time, 2 Gy radiation, or both. Cell viability was determined with the WST-8 assay. The number of colonies was determined with the colony formation assay. mRNA expression of LC3 and caspase-9 was analyzed with quantitative real-time polymerase chain reaction. Results: Combination treatment with EGCG and radiation significantly decreased the growth of HCT-116 cells. The number of colonies was reduced to 34.2% compared to the control group. Immunofluorescence microscopy images showed that nuclear translocation of Nrf2 was significantly increased when cells were treated with the combination of EGCG and radiation compared to the control and single-treatment groups. Combined treatment with EGCG and radiation significantly induced LC3 and caspase-9 mRNA expression. Conclusion: EGCG increased the sensitivity of colorectal cancer cells to radiation by inhibiting cell proliferation and inducing Nrf2 nuclear translocation and autophagy.
掲載誌名
Anticancer Research
ISSN
02507005
17917530
cat書誌ID
AA10625860
AA12440673
出版者
The International Institute of Anticancer Research
38
11
開始ページ
6247
終了ページ
6252
発行日
2018-11-05
備考
内容要旨・審査要旨・論文本文の公開
本論文は, 著者TUMENJIN ENKHBATの学位論文として提出され, 学位審査・授与の対象となっている。
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第3256号
学位記番号
甲医第1405号
学位授与年月日
2019-03-22
学位名
博士(医学)
学位授与機関
徳島大学
部局
医学系
病院