ID | 117354 |
著者 |
Isshiki, Takuma
Toho University
Homma, Sakae
Toho University
Eishi, Yoshinobu
Tokyo Medical and Dental University
Yabe, Matsuko
Toho University
Yamaguchi, Tetsuo
Tokyo Medical and Dental University|Shinjuku Tsurukame Clinic
Uchida, Keisuke
Tokyo Medical and Dental University
Yamamoto, Kurara
Tokyo Medical and Dental University
Ohashi, Kenichi
Tokyo Medical and Dental University
Arakawa, Atsushi
Juntendo University
Shibuya, Kazutoshi
Toho University
Sakamoto, Susumu
Toho University
Kishi, Kazuma
Toho University
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キーワード | sarcoidosis
granuloma
pathogenesis
P. acnes
Propionibacterium acnes
Cutibacterium acnes
immunohistochemistry
PAB antibody
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資料タイプ |
学術雑誌論文
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抄録 | Propionibacterium acnes is implicated in the pathogenesis of sarcoidosis. We investigated the usefulness of immunohistochemistry (IHC) with a commercially available P. acnes-specific monoclonal antibody (PAB antibody) for differentiating sarcoidosis from other granulomatous diseases. Formalin-fixed paraffin-embedded tissue samples from 94 sarcoidosis patients and 30 control patients with other granulomatous diseases were examined by the original manual IHC method. We also compared the detection frequency of P. acnes in sarcoid granulomas between manual and automated IHC methods. P. acnes was detected in sarcoid granulomas of samples obtained by transbronchial lung biopsy (64%), video-associated thoracic surgery (67%), endobronchial-ultrasound-guided transbronchial-needle aspiration (32%), lymph node biopsy (80%), and skin biopsy (80%) from sarcoidosis patients, but not in any non-sarcoid granulomas of the samples obtained from control patients. P. acnes outside granulomas, however, was frequently detected in both groups. The detection status of P. acnes in granulomas did not correlate with the clinical characteristics of sarcoidosis patients. The automated Leica system exhibited the best detection sensitivity (72%) and almost an identical localization for P. acnes in sarcoid granulomas compared with the manual method. IHC with a PAB antibody is useful for differentiating sarcoidosis from other granulomatous diseases by detecting P. acnes in granulomas. An automated method by the Leica system can be used in pathology laboratories for differential diagnosis of granulomas by IHC with the PAB antibody.
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掲載誌名 |
Microorganisms
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ISSN | 20762607
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出版者 | MDPI
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巻 | 9
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号 | 8
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開始ページ | 1668
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発行日 | 2021-08-04
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権利情報 | This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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EDB ID | |
出版社版DOI | |
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言語 |
eng
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著者版フラグ |
出版社版
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部局 |
病院
医学系
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