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ID 117549
著者
Hasegawa, Hijiri LG Japan Lab
Sasaki, Ikkei Tokyo University of Agriculture and Technology
Tsukakoshi, Kaori Tokyo University of Agriculture and Technology
Ma, Yue Tokyo University of Agriculture and Technology
Nagasawa, Kazuo Tokyo University of Agriculture and Technology
Inoue, Yuuki LG Japan Lab
Kim, Yeji LG Japan Lab
Ikebukuro, Kazunori Tokyo University of Agriculture and Technology
キーワード
DNA methylation
G-quadruplex
G4 ligand
biosensor
資料タイプ
学術雑誌論文
抄録
Genomic DNA methylation is involved in many diseases and is expected to be a specific biomarker for even the pre-symptomatic diagnosis of many diseases. Thus, a rapid and inexpensive detection method is required for disease diagnosis. We have previously reported that cytosine methylation in G-quadruplex (G4)-forming oligonucleotides develops different G4 topologies. In this study, we developed a method for detecting CpG methylation in G4-forming oligonucleotides based on the structural differences between methylated and unmethylated G4 DNAs. The differences in G4 topologies due to CpG methylation can be discriminated by G4 ligands. We performed a binding assay between methylated or unmethylated G4 DNAs and G4 ligands. The binding abilities of fluorescent G4 ligands to BCL-2, HRAS1, HRAS2, VEGF G4-forming sequences were examined by fluorescence-based microtiter plate assay. The differences in fluorescence intensities between methylated and unmethylated G4 DNAs were statistically significant. In addition to fluorescence detection, the binding of G4 ligand to DNA was detected by chemiluminescence. A significant difference was also detected in chemiluminescence intensity between methylated and unmethylated DNA. This is the first study on the detection of CpG methylation in G4 structures, focusing on structural changes using G4 ligands.
掲載誌名
International Journal of Molecular Sciences
ISSN
14220067
出版者
MDPI
22
23
開始ページ
13159
発行日
2021-12-06
権利情報
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
医学系