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ID 110154
著者
谷原, 史倫 Laboratory of Animal Reproduction, Faculty of Bioscience and Bioindustry, Tokushima University|Diabetes Therapeutics and Research Center, Tokushima University 徳島大学 教育研究者総覧 KAKEN研究者をさがす
竹本, 龍也 Division of Embryology, Fujii Memorial Institute of Medical Sciences, Tokushima University 徳島大学 教育研究者総覧 KAKEN研究者をさがす
キタガワ, エリ Research and Development Center, NH Foods Ltd
Rao, Shengbin Research and Development Center, NH Foods Ltd
Kim Do, Lanh Thi Laboratory of Animal Reproduction, Faculty of Bioscience and Bioindustry, Tokushima University
オオニシ, アキラ Laboratory of Animal Reproduction, Department of Animal Science and Resources, College of Bioresource Sciences, Nihon University|Transgenic Pig Research Unit, National Institute of Agrobiological Sciences
山下, 裕紀子 Division of Embryology, Fujii Memorial Institute of Medical Sciences, Tokushima University
小杉, 知里 Division of Embryology, Fujii Memorial Institute of Medical Sciences, Tokushima University
鈴木, 仁美 Division of Embryology, Fujii Memorial Institute of Medical Sciences, Tokushima University
センボン, ショウイチロウ Transgenic Pig Research Unit, National Institute of Agrobiological Sciences
スズキ, シュンイチ Transgenic Pig Research Unit, National Institute of Agrobiological Sciences
ナカイ, ミチコ Transgenic Pig Research Unit, National Institute of Agrobiological Sciences
ハシモト, マサカズ Laboratory for Embryogenesis, Graduate School of Frontier Biosciences, Osaka University
泰江, 章博 Department of Orthodontics and Dentofacial Orthopedics, Institute of Biomedical Sciences, Tokushima University Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
松久, 宗英 Diabetes Therapeutics and Research Center, Tokushima University 徳島大学 教育研究者総覧 KAKEN研究者をさがす
野地, 澄晴 Department of Life System, Institute of Technology and Science, Tokushima University 徳島大学 教育研究者総覧 KAKEN研究者をさがす
フジムラ, タツヤ Research and Development Center, NH Foods Ltd
フチモト, ダイイチロウ Transgenic Pig Research Unit, National Institute of Agrobiological Sciences
音井, 威重 Laboratory of Animal Reproduction, Faculty of Bioscience and Bioindustry, Tokushima University 徳島大学 教育研究者総覧 KAKEN研究者をさがす
資料タイプ
学術雑誌論文
抄録
Genetically modified pigs for biomedical applications have been mainly generated using the somatic cell nuclear transfer technique; however, this approach requires complex micromanipulation techniques and sometimes increases the risks of both prenatal and postnatal death by faulty epigenetic reprogramming of a donor somatic cell nucleus. As a result, the production of genetically modified pigs has not been widely applied. We provide a simple method for CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 gene editing in pigs that involves the introduction of Cas9 protein and single-guide RNA into in vitro fertilized zygotes by electroporation. The use of gene editing by electroporation of Cas9 protein (GEEP) resulted in highly efficient targeted gene disruption and was validated by the efficient production of Myostatin mutant pigs. Because GEEP does not require the complex methods associated with micromanipulation for somatic reprogramming, it has the potential for facilitating the genetic modification of pigs.
掲載誌名
Science Advances
ISSN
23752548
2
9
開始ページ
e1600803
並び順
1600803
発行日
2016-9-14
備考
2016 © The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).
EDB ID
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
生物資源系
先端酵素学研究所
病院