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ID 115540
タイトル別表記
ELECTROPORATION OF BOVINE BLASTOCYSTS
著者
Morikawa, Shigeki Tokushima Prefectural Livestock Research Institute
Nguyen, Nhien Thi Tokushima University
Le, Quynh Anh Tokushima University
Hirano, Takayuki Tokushima University
Fukumi, Yoshiyuki Tokushima Prefectural Livestock Research Institute
Abe, Toshiaki Tokushima Prefectural Livestock Research Institute
キーワード
Bovine
Blastocyst
Electroporation
In vivo
Zona pellucida
資料タイプ
学術雑誌論文
抄録
The introduction of exogenous molecules into embryos is required for analyses of molecular dynamics and specific gene functions during early embryonic development. Electroporation is an effective method to transport exogenous molecules into cells, but is rarely used in bovine embryos. First, we evaluated the viability of in vivo-derived bovine blastocysts after electroporation with fluorescein (FAM) labeled-oligonucleotides with varying pulse numbers (3, 5, 7, and 10), while keeping the pulse duration at 1 msec and the electric field of 20 V/mm. Next, we examined the effects of zona pellucida status on blastocyst quality after electroporation, by comparing the average diameter of blastocysts before and after electroporation using blastocysts with intact zona pellucida and hatching/hatched blastocysts. Electroporation successfully introduced exogenous molecules into in vivo-derived bovine blastocysts without loss of viability. Moreover, the status of the zona pellucida may be associated with the quality of blastocysts after electroporation.
掲載誌名
Journal of Reproduction and Development
ISSN
13484400
09168818
cat書誌ID
AA10936678
出版者
Society for Reproduction and Development
65
5
開始ページ
475
終了ページ
479
発行日
2019
権利情報
This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
生物資源系